BMRBj - BMREntryMaster

Found 1 Document (0.819 seconds)

BMRB: 15843

2N49

Solution NMR Structure of the N-Terminal Domain of Protein ECA1580 from Erwinia carotovora, Northeast Structural Genomics Consortium Target EwR156A

Authors

Xiao, R., Lui, J., Mills, J.L., Eletsky, A., Zhang, Q., Lee, D., Jiang, M., Ciccosanti, C., Everett, J.K., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.

Assembly

ECA1580

Entity

1. ECA1580 (polymer, Thiol state: not present), 74 monomers, 8401.418 Da Detail

MAMNGTITTW FKDKGFGFIK DENGDNRYFH VIKVANPDLI KKDAAVTFEP TTNNKGLSAY AVKVVPLEHH HHHH

Formula weight

8401.418 Da

Source organism

Pectobacterium carotovorum

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 88.6 %, Completeness (bb): 90.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (776 of 876)	90.7 % (409 of 451)	85.6 % (297 of 347)	89.7 % (70 of 78)
Backbone	90.0 % (394 of 438)	90.7 % (136 of 150)	89.9 % (195 of 217)	88.7 % (63 of 71)
Sidechain	87.8 % (445 of 507)	90.7 % (273 of 301)	82.9 % (165 of 199)	100.0 % (7 of 7)
Aromatic	64.2 % (68 of 106)	77.4 % (41 of 53)	50.0 % (26 of 52)	100.0 % (1 of 1)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. NC

MAMNGTITTW FKDKGFGFIK DENGDNRYFH VIKVANPDLI KKDAAVTFEP TTNNKGLSAY AVKVVPLEHH HHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	NC	[U-100% 13C; U-100% 15N]	1.07 mM
2	sodium azide	natural abundance	0.02 %
3	DTT	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	sodium chloride	natural abundance	100 mM
6	MES	natural abundance	20 mM
7	H2O	natural abundance	95 %
8	D2O	[U-100% 2H]	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	NC5	[U-5% 13C; U-100% 15N]	1.864 mM
10	sodium azide	natural abundance	0.02 %
11	DTT	natural abundance	100 mM
12	calcium chloride	natural abundance	5 mM
13	sodium chloride	natural abundance	100 mM
14	MES	natural abundance	20 mM
15	H2O	natural abundance	95 %
16	D2O	[U-100% 2H]	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K5N, Strand ID: A Detail

Release date

2009-02-10

Citation

NMR Structure of the Putative Cold Shock Protein from Erwinia carotovora: Northeast Structural Genomics Consortium Target EwR156a
Mills, J.L., Eletsky, A., Zhang, Q., Lee, D., Jiang, M., Ciccosanti, C., Xiao, R., Lui, J., Everett, J.K., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.
Not known

Related entities 1. ECA1580, : 1 : 1 : 42 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC