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BMRB: 15851

2K60

NMR structure of calcium-loaded STIM1 EF-SAM

Authors

Stathopulos, P.B., Ikura, M.

Assembly

Stromal interaction molecule 1

Entity

1. stromal interaction molecule 1 (polymer, Thiol state: not present), 150 monomers, 17357.17 Da Detail

GSHMASSEDE KLSFEAVRNI HKLMDDDANG DVDVEESDEF LREDLNYHDP TVKHSTFHGE DKLISVEDLW KAWKSSEVYN WTVDEVVQWL ITYVELPQYE ETFRKLQLSG HAMPRLAVTN TTMTGTVLKM TDRSHRQKLQ LKALDTVLFG

2. CA (non-polymer), 40.078 Da

Total weight

17397.248 Da

Max. entity weight

17357.17 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 86.5 %, Completeness (bb): 91.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.5 % (1523 of 1760)	89.0 % (808 of 908)	84.5 % (584 of 691)	81.4 % (131 of 161)
Backbone	91.4 % (817 of 894)	92.1 % (279 of 303)	92.1 % (409 of 444)	87.8 % (129 of 147)
Sidechain	83.6 % (844 of 1010)	87.4 % (529 of 605)	80.1 % (313 of 391)	14.3 % (2 of 14)
Aromatic	38.0 % (60 of 158)	65.8 % (52 of 79)	9.3 % (7 of 75)	25.0 % (1 of 4)
Methyl	100.0 % (166 of 166)	100.0 % (83 of 83)	100.0 % (83 of 83)

1. stromal interaction molecule 1

GSHMASSEDE KLSFEAVRNI HKLMDDDANG DVDVEESDEF LREDLNYHDP TVKHSTFHGE DKLISVEDLW KAWKSSEVYN WTVDEVVQWL ITYVELPQYE ETFRKLQLSG HAMPRLAVTN TTMTGTVLKM TDRSHRQKLQ LKALDTVLFG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 8.0

#	Name	Isotope labeling	Concentration
1	stromal interaction molecule 1	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
2	TRIS	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	CALCIUM ION	natural abundance	5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 8.0

#	Name	Isotope labeling	Concentration
5	stromal interaction molecule 1	[U-100% 13C; U-100% 15N]	0.5-1.0 mM
6	TRIS	[U-99% 2H]	20 mM
7	sodium chloride	natural abundance	100 mM
8	CALCIUM ION	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.5 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K60, Strand ID: A Detail

Release date

2008-10-13

Citation

Structural and mechanistic insights into STIM1-mediated initiation of store-operated calcium entry
Stathopulos, P.B., Zheng, L., Li, G., Plevin, M.J., Ikura, M.
Cell (2008), 135, 110-122, PubMed 18854159 , DOI 10.1016/j.cell.2008.08.006 , Abstract

Related entities 1. stromal interaction molecule 1, : 1 : 24 entities Detail

Interaction partners 1. stromal interaction molecule 1, : 32 interactors Detail

More details for 32 interactors identified by 15 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC