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BMRB: 15880

2K6S

Structure of Rab11-FIP2 C-terminal Coiled-coil Domain

Authors

Wei, J., Liu, Y., Baleja, J.D.

Assembly

Rab11-FIP2

Entity

1. Rab11-FIP2 (polymer, Thiol state: not present), 41 monomers, 4995.708 × 2 Da Detail

GSLTYEEVLQ ELVKHKELLR RKDTHIRELE DYIDNLLVRV M

Total weight

9991.416 Da

Max. entity weight

4995.708 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 68.3 %, Completeness (bb): 71.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.3 % (349 of 511)	88.8 % (238 of 268)	37.0 % (74 of 200)	86.0 % (37 of 43)
Backbone	71.5 % (176 of 246)	86.7 % (72 of 83)	55.7 % (68 of 122)	87.8 % (36 of 41)
Sidechain	66.9 % (204 of 305)	89.7 % (166 of 185)	31.4 % (37 of 118)	50.0 % (1 of 2)
Aromatic	45.8 % (11 of 24)	91.7 % (11 of 12)	0.0 % (0 of 12)
Methyl	45.0 % (27 of 60)	83.3 % (25 of 30)	6.7 % (2 of 30)

1. Rab11-FIP2

GSLTYEEVLQ ELVKHKELLR RKDTHIRELE DYIDNLLVRV M

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	entity	natural abundance	1 mM
2	DSS	natural abundance	20 uM
3	sodium phosphate	natural abundance	20 mM
4	D2O	[U-100% 2H]	100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
5	entity	[U-100% 15N]	1.5 mM
6	DSS	natural abundance	20 uM
7	sodium phosphate	natural abundance	20 mM
8	H2O	natural abundance	90 %
9	D2O	[U-100% 2H]	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
10	entity	[U-100% 13C; U-100% 15N]	0.8 mM
11	DSS	natural abundance	20 uM
12	sodium phosphate	natural abundance	20 mM
13	H2O	natural abundance	90 %
14	D2O	[U-100% 2H]	10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 2K6S, Strand ID: A, B Detail

Release date

2009-02-12

Citation

Disorder and structure in the Rab11 binding domain of Rab11 family interacting protein 2
Wei, J., Liu, Y., Bose, K., Henry, G., Baleja, J.D.
Biochemistry (2009), 48, 549-557, PubMed 19119858 , DOI 10.1021/bi8020197 , Abstract

Related entities 1. Rab11-FIP2, : 1 : 9 : 33 entities Detail

Interaction partners 1. Rab11-FIP2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	entity	natural abundance	1 mM
2	DSS	natural abundance	20 uM
3	sodium phosphate	natural abundance	20 mM
4	D2O	[U-100% 2H]	100 %

2 2D 1H-1H COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	entity	natural abundance	1 mM
2	DSS	natural abundance	20 uM
3	sodium phosphate	natural abundance	20 mM
4	D2O	[U-100% 2H]	100 %

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	entity	natural abundance	1 mM
2	DSS	natural abundance	20 uM
3	sodium phosphate	natural abundance	20 mM
4	D2O	[U-100% 2H]	100 %

4 3D HNCO