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BMRB: 15924

2K7P

Filamin A Ig-like domains 16-17

Authors

Heikkinen, O.K., Kilpelainen, I., Koskela, H., Permi, P., Heikkinen, S., Ylanne, J.

Assembly

FLNa16-17

Entity

1. FLNa16-17 (polymer, Thiol state: all free), 188 monomers, 19994.25 Da Detail

GAMAPERPLV GVNGLDVTSL RPFDLVIPFT IKKGEITGEV RMPSGKVAQP TITDNKDGTV TVRYAPSEAG LHEMDIRYDN MHIPGSPLQF YVDYVNCGHV TAYGPGLTHG VVNKPATFTV NTKDAGEGGL SLAIEGPSKA EISCTDNQDG TCSVSYLPVL PGDYSILVKY NEQHVPGSPF TARVTGDD

Formula weight

19994.25 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.8 %, Completeness (bb): 85.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.8 % (1848 of 2057)	98.3 % (1039 of 1057)	77.2 % (630 of 816)	97.3 % (179 of 184)
Backbone	85.5 % (937 of 1096)	99.0 % (377 of 381)	72.2 % (392 of 543)	97.7 % (168 of 172)
Sidechain	95.6 % (1078 of 1128)	97.9 % (662 of 676)	92.0 % (405 of 440)	91.7 % (11 of 12)
Aromatic	79.1 % (106 of 134)	98.5 % (66 of 67)	59.7 % (40 of 67)
Methyl	98.1 % (210 of 214)	100.0 % (107 of 107)	96.3 % (103 of 107)

1. FLNa16-17

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	FLNa16-17	[U-13C; U-15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	potassium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	2 mM
6	EDTA	natural abundance	2 mM
7	H2O	natural abundance	93 %
8	D2O	[U-100% 2H]	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	0.0 ppm	internal	direct	0.2514495
¹H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	water	protons	4.727517 ppm	internal	indirect	0.101329

Referencing offset: -0.12 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	0.0 ppm	internal	direct	0.2514495
¹H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	water	protons	4.727517 ppm	internal	indirect	0.101329

Referencing offset: -0.12 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	0.0 ppm	internal	direct	0.2514495
¹H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	water	protons	4.727517 ppm	internal	indirect	0.101329

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 40 models in PDB: 2K7P, Strand ID: A Detail

Release date

2009-01-14

Citation

1H, 13C and 15N resonance assignments of the human filamin A tandem immunoglobulin-like domains 16-17 and 18-19
Heikkinen, O., Permi, P., Koskela, H., Ylanne, J., Kilpelainen, I.
Biomol. NMR Assign. (2009), 3, 53-56, PubMed 19636946 , DOI 10.1007/s12104-008-9140-6 , Abstract

Related entities 1. FLNa16-17, : 1 : 2 : 195 entities Detail

Interaction partners 1. FLNa16-17, : 141 interactors Detail

More details for 141 interactors identified by 56 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC