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BMRB: 15925

2K7Q

Filamin A Ig-like domains 18-19

Authors

Heikkinen, O.K., Kilpelainen, I., Koskela, H., Permi, P., Heikkinen, S., Ylanne, J.

Assembly

FLNa18-19

Entity

1. FLNa18-19 (polymer, Thiol state: all free), 191 monomers, 20379.71 Da Detail

GAMGDDSMRM SHLKVGSAAD IPINISETDL SLLTATVVPP SGREEPCLLK RLRNGHVGIS FVPKETGEHL VHVKKNGQHV ASSPIPVVIS QSEIGDASRV RVSGQGLHEG HTFEPAEFII DTRDAGYGGL SLSIEGPSKV DINTEDLEDG TCRVTYCPTE PGNYIINIKF ADQHVPGSPF SVKVTGEGRV K

Formula weight

20379.71 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.8 %, Completeness (bb): 94.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.8 % (2011 of 2099)	98.0 % (1064 of 1086)	93.7 % (773 of 825)	92.6 % (174 of 188)
Backbone	94.3 % (1056 of 1120)	96.2 % (375 of 390)	93.7 % (517 of 552)	92.1 % (164 of 178)
Sidechain	96.5 % (1109 of 1149)	97.6 % (679 of 696)	94.8 % (420 of 443)	100.0 % (10 of 10)
Aromatic	84.9 % (90 of 106)	100.0 % (53 of 53)	69.8 % (37 of 53)
Methyl	99.1 % (214 of 216)	100.0 % (108 of 108)	98.1 % (106 of 108)

1. FLNa18-19

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	FLNa18-19	[U-13C; U-15N]	0.8 mM
2	sodium phosphate	natural abundance	50 mM
3	potassium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	2 mM
6	H2O	natural abundance	93 %
7	D2O	[U-100% 2H]	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.727517 ppm	internal	indirect	0.2514495
¹H	water	protons	4.727517 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.727517 ppm	internal	indirect	0.101329

Referencing offset: 0.29 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.727517 ppm	internal	indirect	0.2514495
¹H	water	protons	4.727517 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.727517 ppm	internal	indirect	0.101329

Referencing offset: 0.29 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.727517 ppm	internal	indirect	0.2514495
¹H	water	protons	4.727517 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.727517 ppm	internal	indirect	0.101329

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.727517 ppm	internal	indirect	0.2514495
¹H	water	protons	4.727517 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.727517 ppm	internal	indirect	0.101329

Referencing offset: 0.47 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K7Q, Strand ID: A Detail

Release date

2009-01-14

Citation

1H, 13C and 15N resonance assignments of the human filamin A tandem immunoglobulin-like domains 16-17 and 18-19
Heikkinen, O., Permi, P., Koskela, H., Ylanne, J., Kilpelainen, I.
Biomol. NMR Assign. (2009), 3, 53-56, PubMed 19636946 , DOI 10.1007/s12104-008-9140-6 , Abstract

Related entities 1. FLNa18-19, : 1 : 5 : 268 entities Detail

Interaction partners 1. FLNa18-19, : 141 interactors Detail

More details for 141 interactors identified by 55 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC