BMRBj - BMREntryMaster

Found 1 Document (0.676 seconds)

BMRB: 15943

2K8E

Solution NMR Structure of protein of unknown function yegP from E. coli. Ontario Center for Structural Proteomics target EC0640_1_123 Northeast Structural Genomics Consortium Target ET102.

Authors

Fares, C., Lemak, A., Gutmanas, A., Karra, M., Yee, A.H., Semesi, A., Arrowsmith, C.H.

Assembly

yegP

Entity

1. yegP (polymer, Thiol state: not present), 116 monomers, 12551.72 Da Detail

GVIMAGWFEL SKSSDNQFRF VLKAGNGETI LTSELYTSKT SAEKGIASVR SNSPQEERYE KKTASNGKFY FNLKAANHQI IGSSQMYATA QSRETGIASV KANGTSQTVK DNTGSN

Formula weight

12551.72 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.0 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.0 % (1175 of 1305)	90.3 % (611 of 677)	89.1 % (443 of 497)	92.4 % (121 of 131)
Backbone	97.1 % (674 of 694)	97.9 % (236 of 241)	96.4 % (326 of 338)	97.4 % (112 of 115)
Sidechain	84.2 % (604 of 717)	86.0 % (375 of 436)	83.0 % (220 of 265)	56.3 % (9 of 16)
Aromatic	57.1 % (56 of 98)	71.4 % (35 of 49)	41.7 % (20 of 48)	100.0 % (1 of 1)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. protein of unkown function yegP from E.coli

GVIMAGWFEL SKSSDNQFRF VLKAGNGETI LTSELYTSKT SAEKGIASVR SNSPQEERYE KKTASNGKFY FNLKAANHQI IGSSQMYATA QSRETGIASV KANGTSQTVK DNTGSN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	EC0640	[U-100% 13C; U-100% 15N]	0.4-0.7 mM
2	tris		10 mM
3	NaCl		300 mM
4	benzamidine		1 mM
5	NaN3		0.01 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K8E, Strand ID: A Detail

Release date

2009-04-22

Citation

Solution structure of protein yegP from Escherichia Coli
Fares, C., Lemak, A., Gutmanas, A., Karra, M., Yee, A., Semesi, A., Arrowsmith, C.H.
Not known

Related entities 1. yegP, : 1 : 4 : 11 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC