BMRBj - BMREntryMaster

Found 1 Document (5.838 seconds)

BMRB: 15944

2K8F

Structural Basis for the Regulation of p53 Function by p300

Authors

Bai, Y., Feng, H., Jenkins, L.M., Durell, S.R., Wiodawer, A., Appella, E.

Assembly

Taz2-p53 TAD1

Entity

1. TAZ2 (polymer, Thiol state: all free), 90 monomers, 9943.619 Da Detail

ATQSPGDSRR LSIQRAIQSL VHAAQCRNAN CSLPSCQKMK RVVQHTKGCK RKTNGGCPIC KQLIALAAYH AKHCQENKCP VPFCLNIKQK

2. TAD(1-39) (polymer, Thiol state: all free), 39 monomers, 4350.760 Da Detail

MEEPQSDPSV EPPLSQETFS DLWKLLPENN VLSPLPSQA

Total weight

14294.379 Da

Max. entity weight

9943.619 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 84.9 %, Completeness (bb): 94.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (1277 of 1504)	82.2 % (657 of 799)	89.4 % (508 of 568)	81.8 % (112 of 137)
Backbone	94.5 % (709 of 750)	94.0 % (235 of 250)	94.8 % (363 of 383)	94.9 % (111 of 117)
Sidechain	78.5 % (690 of 879)	76.9 % (422 of 549)	86.1 % (267 of 310)	5.0 % (1 of 20)
Aromatic	0.0 % (0 of 56)	0.0 % (0 of 28)	0.0 % (0 of 27)	0.0 % (0 of 1)
Methyl	87.5 % (105 of 120)	86.7 % (52 of 60)	88.3 % (53 of 60)

1. TAZ2

ATQSPGDSRR LSIQRAIQSL VHAAQCRNAN CSLPSCQKMK RVVQHTKGCK RKTNGGCPIC KQLIALAAYH AKHCQENKCP VPFCLNIKQK

2. TAD(1-39)

MEEPQSDPSV EPPLSQETFS DLWKLLPENN VLSPLPSQA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and/or 13C labeled p53(1-39) with non-labeled TAZ2 complex in 50 mM MES, 200 mM NaCl, 0.1 mM ZnCl, 1mM DTT, pH 6.3.

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
7	TAZ2	[U-100% 15N]	1.0-1.2 mM
8	TAD(1-39)	natural abundance	1.1-1.3 mM
9	MES	natural abundance	50 mM
10	NaCl	natural abundance	200 mM
11	ZnCl	natural abundance	0.1 mM
12	DTT	natural abundance	1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details non-labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
19	TAZ2	natural abundance	1.0-1.2 mM
20	TAD(1-39)	natural abundance	1.1-1.3 mM
21	MES	natural abundance	50 mM
22	NaCl	natural abundance	200 mM
23	ZnCl	natural abundance	0.1 mM
24	DTT	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.33 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.33 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2K8F, Strand ID: A, B Detail

Release date

2009-03-11

Citation

Structural basis for p300 Taz2-p53 TAD1 binding and modulation by phosphorylation
Feng, H., Miller Jenkins, L.M., Durell, S.R., Hayashi, R., Mazur, S.J., Cherry, S., Tropea, J.E., Miller, M., Wlodawer, A., Appella, E., Bai, Y.
Structure (2009), 17, 202-210, PubMed 19217391 , DOI 10.1016/j.str.2008.12.009 , Abstract

Related entities 1. TAZ2, : 1 : 9 : 10 : 56 entities Detail

Related entities 2. TAD(1-39), : 1 : 9 : 2 : 1 : 18 entities Detail

Interaction partners 1. TAZ2, : 5 : 1 interactors Detail

More details for 6 interactors identified by 6 citations

Interaction partners 2. TAD(1-39), : 374 : 1 interactors Detail

More details for 375 interactors identified by 181 citations

Experiments performed 22 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
7	TAZ2	[U-100% 15N]	1.0-1.2 mM
8	TAD(1-39)	natural abundance	1.1-1.3 mM
9	MES	natural abundance	50 mM
10	NaCl	natural abundance	200 mM
11	ZnCl	natural abundance	0.1 mM
12	DTT	natural abundance	1 mM

2 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details non-labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
19	TAZ2	natural abundance	1.0-1.2 mM
20	TAD(1-39)	natural abundance	1.1-1.3 mM
21	MES	natural abundance	50 mM
22	NaCl	natural abundance	200 mM
23	ZnCl	natural abundance	0.1 mM
24	DTT	natural abundance	1 mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

4 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

5 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

6 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

7 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

8 3D H(CCO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

10 3D HNHA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
7	TAZ2	[U-100% 15N]	1.0-1.2 mM
8	TAD(1-39)	natural abundance	1.1-1.3 mM
9	MES	natural abundance	50 mM
10	NaCl	natural abundance	200 mM
11	ZnCl	natural abundance	0.1 mM
12	DTT	natural abundance	1 mM

11 3D 1H-15N NOESY

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
7	TAZ2	[U-100% 15N]	1.0-1.2 mM
8	TAD(1-39)	natural abundance	1.1-1.3 mM
9	MES	natural abundance	50 mM
10	NaCl	natural abundance	200 mM
11	ZnCl	natural abundance	0.1 mM
12	DTT	natural abundance	1 mM

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and 13C labeled TAZ2 and non-labeled TAD(1-39) complex.

#	Name	Isotope labeling	Concentration
13	TAZ2	[U-100% 13C; U-100% 15N]	1.0-1.2 mM
14	TAD(1-39)	natural abundance	1.1-1.3 mM
15	MES	natural abundance	50 mM
16	NaCl	natural abundance	200 mM
17	ZnCl	natural abundance	0.1 mM
18	DTT	natural abundance	1 mM

13 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3, Details 15N and/or 13C labeled p53(1-39) with non-labeled TAZ2 complex in 50 mM MES, 200 mM NaCl, 0.1 mM ZnCl, 1mM DTT, pH 6.3.

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

14 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

15 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

16 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

17 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

18 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

19 3D H(CCO)NH

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

20 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

21 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

22 3D 1H-13C NOESY

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TAZ2	natural abundance	1.1-1.2 mM
2	TAD(1-39)	[U-100% 15N]	1.0-1.1 mM
3	MES	natural abundance	50 mM
4	NaCl	natural abundance	200 mM
5	ZnCl	natural abundance	0.1 mM
6	DTT	natural abundance	1 mM

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_15944_2k8f.nef
Input source #2: Coordindates	2k8f.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90
ATQSPGDSRRLSIQRAIQSLVHAAQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALAAYHAKHCQENKCPVPFCLNIKQK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ATQSPGDSRRLSIQRAIQSLVHAAQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALAAYHAKHCQENKCPVPFCLNIKQK

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------10--------20--------30---------
MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQA
|||||||||||||||||||||||||||||||||||||||
MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQA

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	90	0	0	100.0
B	B	39	0	0	100.0

Content subtype: combined_15944_2k8f.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1266		98.9 (chain: A, length: 90), 94.9 (chain: B, length: 39)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	704 (1)	noe	95.6 (chain: A, length: 90), 59.0 (chain: B, length: 39)
2	Distance restraints	CNS/XPLOR_distance_constraints_7	Warning	1517 (1)	noe	98.9 (chain: A, length: 90), 92.3 (chain: B, length: 39)
3	Distance restraints	CNS/XPLOR_distance_constraints_5	Warning	94 (1)	hbond	66.7 (chain: A, length: 90), 20.5 (chain: B, length: 39)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	136 (136)	.	82.2 (chain: A, length: 90), 41.0 (chain: B, length: 39)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 90, Sequence coverage: 98.9%
  - Entity_assembly_ID: B, Chain length: 39, Sequence coverage: 94.9%
- Total number of assignments
  - ¹³C chemical shifts: 506
  - ¹H chemical shifts: 649
  - ¹⁵N chemical shifts: 111
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	391	356	91.0
¹H chemical shifts	557	464	83.3
¹⁵N chemical shifts	105	83	79.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	180	169	93.9
¹H chemical shifts	179	174	97.2
¹⁵N chemical shifts	85	83	97.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	211	187	88.6
¹H chemical shifts	378	290	76.7
¹⁵N chemical shifts	20	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	43	38	88.4
¹H chemical shifts	43	37	86.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	17	0	0.0
¹H chemical shifts	17	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	177	150	84.7
¹H chemical shifts	242	185	76.4
¹⁵N chemical shifts	38	28	73.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	78	71	91.0
¹H chemical shifts	71	63	88.7
¹⁵N chemical shifts	32	28	87.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	99	79	79.8
¹H chemical shifts	171	122	71.3
¹⁵N chemical shifts	6	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	19	14	73.7
¹H chemical shifts	19	14	73.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	10	0	0.0
¹H chemical shifts	11	0	0.0
¹⁵N chemical shifts	1	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 90, Sequence coverage: 95.6%
  - Entity_assembly_ID: B, Chain length: 39, Sequence coverage: 59.0%
- Total number of restraints: 704
- Weight of restraints: 1.0 (704)
- Potential type of restraints: square-well-parabolic (704)
- Range of distance target values: 2.35, 5.15 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_7
  - Status: Warning
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 90, Sequence coverage: 98.9%
    - Entity_assembly_ID: B, Chain length: 39, Sequence coverage: 92.3%
  - Total number of restraints: 1517
  - Weight of restraints: 1.0 (1517)
  - Potential type of restraints: square-well-parabolic (1517)
  - Range of distance target values: 2.4, 9.25 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map
  - Saveframe: CNS/XPLOR_distance_constraints_5
    - Status: Warning
    - Experiment type: hbond
    - Sequence coverage of experimental data
      - Entity_assembly_ID: A, Chain length: 90, Sequence coverage: 66.7%
      - Entity_assembly_ID: B, Chain length: 39, Sequence coverage: 20.5%
    - Total number of restraints: 94
    - Weight of restraints: 1.0 (94)
    - Potential type of restraints: square-well-parabolic (94)
    - Range of distance target values: 1.9, 2.75 (Å)
    - Histogram of distance target values
    - Distance restraints per residue
      - Chain_ID: A
      - Chain_ID: B
    - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 90, Sequence coverage: 82.2%
  - Entity_assembly_ID: B, Chain length: 39, Sequence coverage: 41.0%
- Total number of restraints: 136
- Total number of restraints per polymer type: 136
- Weight of restraints: 1.0 (136)
- Potential type of restraints: square-well-parabolic (136)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Complex of p53 and p300

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (5.838 seconds)

BMRB: 15944

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. TAZ2, : 1 : 9 : 10 : 56 entities Detail

Related entities 2. TAD(1-39), : 1 : 9 : 2 : 1 : 18 entities Detail

Interaction partners 1. TAZ2, : 5 : 1 interactors Detail

Interaction partners 2. TAD(1-39), : 374 : 1 interactors Detail

Experiments performed 22 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample