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BMRB: 15948

2KIZ

1H, 13C, and 15N Chemical Shift Assignments for the RING finger of the E3 ubiquitin ligase Arkadia (RNF111)

Authors

Kandias, N.G., Chasapis, C.T., Episkopou, V., Bentrop, D., Spyroulias, G.A.

Assembly

RING Finger monomer

Entity

1. E3 ubiquitin ligase Arkadia (polymer, Thiol state: all other bound), 69 monomers, 7950.958 Da Detail

MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RRLPCMHLFH QVCVDQWLIT NKKCPICRVD IEAQLPSES

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8081.776 Da

Max. entity weight

7950.958 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS17:SG	2:ZN1:ZN
2	na	sing	1:CYS20:SG	2:ZN1:ZN
3	na	sing	1:HIS40:ND1	2:ZN1:ZN
4	na	sing	1:CYS43:SG	2:ZN1:ZN
5	na	sing	1:CYS35:SG	2:ZN1:ZN
6	na	sing	1:HIS37:ND1	2:ZN1:ZN
7	na	sing	1:CYS54:SG	2:ZN1:ZN
8	na	sing	1:CYS57:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 94.6 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.6 % (753 of 796)	94.7 % (396 of 418)	94.8 % (290 of 306)	93.1 % (67 of 72)
Backbone	95.8 % (391 of 408)	96.4 % (133 of 138)	95.6 % (195 of 204)	95.5 % (63 of 66)
Sidechain	94.1 % (427 of 454)	93.9 % (263 of 280)	95.2 % (160 of 168)	66.7 % (4 of 6)
Aromatic	93.3 % (28 of 30)	100.0 % (15 of 15)	85.7 % (12 of 14)	100.0 % (1 of 1)
Methyl	98.6 % (69 of 70)	100.0 % (35 of 35)	97.1 % (34 of 35)

1. E3 ubiquitin ligase Arkadia

MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RRLPCMHLFH QVCVDQWLIT NKKCPICRVD IEAQLPSES

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	E3 ubiquitin ligase Arkadia	[U-98% 13C; U-98% 15N]		1.0 (±0.2) mM
2	Pi			50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.35 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 31 models in PDB: 2KIZ, Strand ID: A Detail

Release date

2009-01-15

Citation

High yield expression and NMR characterization of Arkadia E3 ubiquitin ligase RING-H2 finger domain
Kandias, N.G., Chasapis, C.T., Bentrop, D., Episkopou, V., Spyroulias, G.A.
Biochem. Biophys. Res. Commun. (2009), 378, 498-502, PubMed 19032943 , DOI 10.1016/j.bbrc.2008.11.055 , Abstract

Related entities 1. E3 ubiquitin ligase Arkadia, : 1 : 9 : 126 entities Detail

Interaction partners 1. E3 ubiquitin ligase Arkadia, : 38 interactors Detail

More details for 38 interactors identified by 11 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC