BMRB: 15965

Structural Transitions of the N Terminus of a Short Non-Muscle Tropomyosin upon Binding to the C Terminus in Solution

Authors

Greenfield, N.J., Kotylanskaya, L., Hitchcock-DeGregori, S.E.

Assembly

TM1b(1-19)Zip/TM9d252-284 complex

Entity

1. TM1b(1-19)Zip (polymer, Thiol state: not present), 38 monomers, 4322.841 Da Detail

GAGSSSLEAV RRKIRSLQEQ NYHLENEVAR LKKLVGER

2. TM9d(252-284) (polymer, Thiol state: all disulfide bound), 37 monomers, 4114.568 Da Detail

GCGGSIDDLE EKVAHAKEEN LSMHQMLDQT LLELNNM

Total weight

8437.408 Da

Max. entity weight

4322.841 Da

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 50.7 %, Completeness: 46.3 %, Completeness (bb): 42.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	46.3 % (397 of 858)	50.2 % (227 of 452)	39.8 % (128 of 322)	50.0 % (42 of 84)
Backbone	42.9 % (193 of 450)	49.4 % (77 of 156)	35.6 % (78 of 219)	50.7 % (38 of 75)
Sidechain	50.1 % (239 of 477)	50.7 % (150 of 296)	49.4 % (85 of 172)	44.4 % (4 of 9)
Aromatic	25.0 % (5 of 20)	50.0 % (5 of 10)	0.0 % (0 of 10)
Methyl	52.5 % (42 of 80)	52.5 % (21 of 40)	52.5 % (21 of 40)

1. TM1b(1-19)Zip

GAGSSSLEAV RRKIRSLQEQ NYHLENEVAR LKKLVGER

2. TM9d(252-284)

GCGGSIDDLE EKVAHAKEEN LSMHQMLDQT LLELNNM

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.1) K, pH 6.4, Details 13C, 15N TM1b(1-19)Zip + umlabled TM9d(252-284) 100 mM NaCl, 10 mM sodium phosphate, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	TM1b(1-14)Zip	[U-99% 13C; U-99% 15N]		1 (±0.1) mM
2	TM9d(252-284)	natural abundance		1 (±0.1) mM
3	NaCl	natural abundance		100 mM
4	sodium phosphate	natural abundance		10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.1) K, pH 6.4, Details 15N TM1b(1-19)Zip + umlabeled TM9d(252-284) 100 mM NaCl, 10 mM sodium phosphate, pH 6.4

#	Name	Isotope labeling	Type	Concentration
5	TM1b(1-19)Zip	[U-15N]		1 (±0.1) mM
6	TM9d(252-284)	natural abundance		1 (±0.1) mM
7	NaCl	natural abundance		100 mM
8	sodium phosphate	natural abundance		10 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.1) K, pH 6.4, Details heterodimer of 13C, 15N TM1b(1-19)Zip + unlabeled TM1b(1-19)Zip and unlabeled TM9d(252-284) 100 mM NaCl, 10 mM sodium phosphate, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	TM1b(1-19)Zip	[U-99% 13C; U-99% 15N]		0.5 (±0.05) mM
10	TM1b(1-19)Zip	natural abundance		0.5 (±0.05) mM
11	TM9d(252-284)	natural abundance		1 (±0.1) mM
12	NaCl	natural abundance		100 mM
13	sodium phosphate	natural abundance		10 mM

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 2K8X, Strand ID: A, B Detail

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Release date

2009-04-01

Citation

Structure of the N terminus of a nonmuscle alpha-tropomyosin in complex with the C terminus: implications for actin binding
Greenfield, N.J., Kotlyanskaya, L., Hitchcock-DeGregori, S.E.
Biochemistry (2009), 48, 1272-1283, PubMed 19170537 , DOI 10.1021/bi801861k , Abstract

Related entities 1. TM1b(1-19)Zip, : 38 entities Detail

Related entities 2. TM9d(252-284), : 22 entities Detail

Experiments performed 15 experiments Detail