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BMRB: 15981

2K8Y

Solution NMR Structure of Cgi121 from Methanococcus jannaschii. Northeast Structural Genomics Consortium Target MJ0187

Authors

Rumpel, S., Fares, C., Neculai, D., Arrowsmith, C., Sicheri, F.

Assembly

Cgi121

Entity

1. Cgi121 (polymer, Thiol state: all free), 150 monomers, 17063.83 Da Detail

GAMDPMIIRG IRGARINNEI FNLGLKFQIL NADVVATKKH VLHAINQAKT KKPIAKSFWM EILVRASGQR QIHEAIKIIG AKDGNVCLIC EDEETFRKIY ELIGGEIDDS VLEINEDKER LIREIFKIRG FGNVVERVLE KIALIELKKE

Formula weight

17063.83 Da

Source organism

Methanocaldococcus jannaschii DSM 2661

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (1766 of 1823)	96.1 % (920 of 957)	97.9 % (690 of 705)	96.9 % (156 of 161)
Backbone	98.8 % (885 of 896)	98.1 % (303 of 309)	99.8 % (438 of 439)	97.3 % (144 of 148)
Sidechain	95.5 % (1018 of 1066)	95.1 % (616 of 648)	96.3 % (390 of 405)	92.3 % (12 of 13)
Aromatic	84.8 % (78 of 92)	84.8 % (39 of 46)	84.4 % (38 of 45)	100.0 % (1 of 1)
Methyl	99.0 % (202 of 204)	98.0 % (100 of 102)	100.0 % (102 of 102)

1. Cgi121

GAMDPMIIRG IRGARINNEI FNLGLKFQIL NADVVATKKH VLHAINQAKT KKPIAKSFWM EILVRASGQR QIHEAIKIIG AKDGNVCLIC EDEETFRKIY ELIGGEIDDS VLEINEDKER LIREIFKIRG FGNVVERVLE KIALIELKKE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2K8Y, Strand ID: A Detail

Release date

2009-02-08

Citation

Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine
Mao, D.Y., Neculai, D., Downey, M., Orlicky, S., Haffani, Y., Ceccarelli, D.F., Ho, J.S., Szilard, R.K., Zhang, W., Ho, C.S., Wan, L., Fares, C., Rumpel, S., Kurinov, I., Durocher, D., Sicheri, F.
Mol. Cell (2008), 32, 259-275, PubMed 18951093 , DOI 10.1016/j.molcel.2008.10.002 , Abstract

Entries sharing articles Swiss-Prot: 4 entries Detail

  Swiss-Prot: Q9HLA5 released on 2007-09-11
    Title KAE1B_THEAC Entity Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
  Swiss-Prot: Q9Y3C4 released on 2007-03-06
    Title TPRKB_HUMAN Entity EKC/KEOPS complex subunit TPRKB
  Swiss-Prot: Q58530 released on 1998-07-15
    Title KAE1B_METJA Entity Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
  Swiss-Prot: Q57646 released on 1997-11-01
    Title CG121_METJA Entity Regulatory protein Cgi121

Related entities 1. Cgi121, : 1 : 2 : 2 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC