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Found 1 Document (1.361 seconds)

BMRB: 15996

2WCY

1H, 15N and 13C resonance assignment of the pair of Factor-I like modules of the complement protein C7

Authors

Phelan, M.M., Thai, C., Herbert, A.P., Bella, J., Uhrin, D., Ogata, R.T., Barlow, P.N., Bramham, J.

Assembly

complement component C7

Entity

1. complement component C7 (polymer, Thiol state: all disulfide bound), 155 monomers, 16955.30 Da Detail

GSHMNPLTQA VPKCQRWEKL QNSRCVCKMP YECGPSLDVC AQDERSKRIL PLTVCKMHVL HCQGRNYTLT GRDSCTLPAS AEKACGACPL WGKCDAESSK CVCREASECE EEGFSICVEV NGKEQTMSEC EAGALRCRGQ SISVTSIRPC AAETQ

Formula weight

16955.3 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEAILING, RESTRAINED MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 92.2 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.2 % (1564 of 1697)	92.4 % (822 of 890)	91.3 % (590 of 646)	94.4 % (152 of 161)
Backbone	94.4 % (863 of 914)	95.5 % (298 of 312)	93.6 % (426 of 455)	94.6 % (139 of 147)
Sidechain	90.5 % (840 of 928)	90.8 % (525 of 578)	89.9 % (302 of 336)	92.9 % (13 of 14)
Aromatic	87.1 % (54 of 62)	87.1 % (27 of 31)	86.2 % (25 of 29)	100.0 % (2 of 2)
Methyl	95.5 % (126 of 132)	95.5 % (63 of 66)	95.5 % (63 of 66)

1. C7-FIMs

GSHMNPLTQA VPKCQRWEKL QNSRCVCKMP YECGPSLDVC AQDERSKRIL PLTVCKMHVL HCQGRNYTLT GRDSCTLPAS AEKACGACPL WGKCDAESSK CVCREASECE EEGFSICVEV NGKEQTMSEC EAGALRCRGQ SISVTSIRPC AAETQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
4	potassium phosphate	natural abundance		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.766 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.766 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.766 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.766 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 48 models in PDB: 2WCY, Strand ID: A Detail

Release date

2009-01-14

Citation

1H, 15N and 13C resonance assignment of the pair of Factor-I like modules of the complement protein C7
Phelan, M.M., Thai, C., Herbert, A.P., Bella, J., Uhrin, D., Ogata, R.T., Barlow, P.N., Bramham, J.
Biomol. NMR Assign. (2009), 3, 49-52, PubMed 19636945 , DOI 10.1007/s12104-008-9139-z , Abstract

Related entities 1. complement component C7, : 1 : 6 : 30 entities Detail

Interaction partners 1. complement component C7, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
4	potassium phosphate	natural abundance		20 mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

5 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

6 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

8 3D HBHANH

Instrument

Bruker Avance - 600 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
4	potassium phosphate	natural abundance		20 mM

10 3D HNCO

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

11 3D HN(CA)CO

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
2	potassium phosphate	natural abundance		20 mM

12 2D (HB)CB(CGCD)HD

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
4	potassium phosphate	natural abundance		20 mM

13 2D (HB)CB(CGCDCE)HE

Instrument

Bruker Avance - 800 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	C7-FIMs	[U-98% 13C; U-98% 15N]		0.2-0.3 mM
4	potassium phosphate	natural abundance		20 mM

nullKeywords C7, complement, factor I module, FIM, membrane attack complex, NMR, resonance assignment

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Found 1 Document (1.361 seconds)

BMRB: 15996

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. complement component C7, : 1 : 6 : 30 entities Detail

Interaction partners 1. complement component C7, : 3 interactors Detail

Experiments performed 13 experiments Detail

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