BMRB: 16020

Magic-Angle-Spinning Solid-State NMR Structure of Influenza A M2 Transmembrane Domain

Authors

Hong, M., Cady, S.D., Mishanina, T.V.

Assembly

M2 Transmembrane Peptide

Entity

1. M2 Transmembrane Peptide (polymer, Thiol state: not present), 25 monomers, 2686.196 × 4 Da Detail

SSDPLVVAAS IIGILHLIAW ILDRL

2. 308 (non-polymer), 151.249 Da

Total weight

10896.033 Da

Max. entity weight

2686.196 Da

Source organism

Alphainfluenzavirus

Exptl. method

solid-state NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 44.0 %, Completeness: 43.1 %, Completeness (bb): 43.9 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	43.1 % (62 of 144)	42.9 % (51 of 119)	44.0 % (11 of 25)
Backbone	43.9 % (43 of 98)	43.2 % (32 of 74)	45.8 % (11 of 24)
Sidechain	41.4 % (29 of 70)	42.0 % (29 of 69)	0.0 % (0 of 1)
Aromatic	0.0 % (0 of 8)	0.0 % (0 of 7)	0.0 % (0 of 1)
Methyl	59.3 % (16 of 27)	59.3 % (16 of 27)

1. M2 Transmembrane Peptide

SSDPLVVAAS IIGILHLIAW ILDRL

Show sample condition

Sample

Solvent system Solid-State NMR: Hydrated DLPC gel, approx. 50% H2O by weight. DLPC:M2TMP:Amantadine 15:1:8, Pressure 1 atm, Temperature 243 K, pH 7.5, Details Solid-State NMR: DLPC:M2TMP:amantadine 15:1:8, 10 mM phosphate buffer, 1 mM EDTA, 0.1 mM NaN3, pH 7.5.

#	Name	Isotope labeling	Type	Concentration
1	M2 Transmembrane Peptide	Selective U-13C, 15N labeled		4.0 ~ 6.0 mg
2	Amantadine	natural abundance		15.0 ~ 25.0 mg
3	DLPC	natural abundance		15.0 ~ 25.0 mg
4	sodium phosphate	natural abundance		10 mM
5	sodium azide	natural abundance		.1 mM
6	EDTA	natural abundance		1 mM
7	H2O	natural abundance		20.0 ~ 25.0 mg

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Protein Blocks Logo

Calculated from 1 models in PDB: 2KAD, Strand ID: A, B, C, D Detail

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Release date

2009-02-03

Citation 1

Structure of amantadine-bound M2 transmembrane peptide of influenza A in lipid bilayers from magic-angle-spinning solid-state NMR: the role of Ser31 in amantadine binding
Cady, S.D., Mishanina, T.V., Hong, M.
J. Mol. Biol. (2009), 385, 1127-1141, PubMed 19061899 , DOI 10.1016/j.jmb.2008.11.022 , Abstract

Show more 3 citaions

Citation 2

Amantadine-induced conformational and dynamical changes of the influenza M2 transmembrane proton channel
Cady, S.D., Hong, M.
Proc. Natl. Acad. Sci. U. S. A. (2008), 105, 1483-1488, PubMed 18230730 , DOI 10.1073/pnas.0711500105 , Abstract

Citation 3

Side-chain conformation of the M2 transmembrane peptide proton channel of influenza a virus from 19F solid-state NMR
Luo, W., Mani, R., Hong, M.
J. Phys. Chem. B (2007), 111, 10825-10832, PubMed 17705425 , DOI 10.1021/jp073823k , Abstract

Citation 4

Determination of the oligomeric number and intermolecular distances of membrane protein assemblies by anisotropic 1H-driven spin diffusion NMR spectroscopy
Luo, W., Hong, M.
J. Am. Chem. Soc. (2006), 128, 7242-7251, PubMed 16734478 , DOI 10.1021/ja0603406 , Abstract

Hide non-primary 3 citaions

Related entities 1. M2 Transmembrane Peptide, : 1 : 66 : 62 entities Detail

Interaction partners 1. M2 Transmembrane Peptide, : 12 interactors Detail

Experiments performed 4 experiments Detail

NMR combined restraints 2 contents Detail