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BMRB: 16029

2N4F

Solution NMR structure of ubiquitin-like domain of Arabidopsis thaliana protein At2g32350. Northeast Structural Genomics Consortium target AR3433A

Authors

Eletsky, A., Mills, J.L., Sukumaran, D., Hua, J., Shastry, R., Jiang, M., Ciccosanti, C., Xiao, R., Liu, J., Everret, J.K., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.

Assembly

At2g32350

Entity

1. At2g32350 (polymer, Thiol state: not present), 94 monomers, 10983.34 Da Detail

MGHHHHHHSH AAVRKIHVTV KFPSKQFTVE VDRTETVSSL KDKIHIVENT PIKRMQLYYS GIELADDYRN LNEYGITEFS EIVVFLKSIN RAKD

Formula weight

10983.34 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.4 %, Completeness: 87.5 %, Completeness (bb): 89.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.5 % (990 of 1131)	88.4 % (518 of 586)	85.9 % (384 of 447)	89.8 % (88 of 98)
Backbone	89.5 % (501 of 560)	88.4 % (167 of 189)	90.3 % (252 of 279)	89.1 % (82 of 92)
Sidechain	86.4 % (572 of 662)	88.4 % (351 of 397)	83.0 % (215 of 259)	100.0 % (6 of 6)
Aromatic	51.9 % (56 of 108)	64.8 % (35 of 54)	38.9 % (21 of 54)
Methyl	99.1 % (107 of 108)	100.0 % (54 of 54)	98.1 % (53 of 54)

1. ar3433a protein

MGHHHHHHSH AAVRKIHVTV KFPSKQFTVE VDRTETVSSL KDKIHIVENT PIKRMQLYYS GIELADDYRN LNEYGITEFS EIVVFLKSIN RAKD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	ar3433a protein	[U-100% 13C; U-100% 15N]	1.09 mM
2	DSS	natural abundance	50 uM
3	sodium chloride	natural abundance	200 mM
4	MES	natural abundance	20 mM
5	calcium cloride	natural abundance	5 mM
6	DTT	natural abundance	10 mM
7	sodium azide	natural abundance	0.02 %
8	protease inhibitors	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
11	ar3433a protein	[U-5% 13C; U-100% 15N]	1.15 mM
12	DSS	natural abundance	50 uM
13	sodium chloride	natural abundance	200 mM
14	MES	natural abundance	20 mM
15	calcium cloride	natural abundance	5 mM
16	DTT	natural abundance	10 mM
17	sodium azide	natural abundance	0.02 %
18	protease inhibitors	natural abundance	1 na
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KAN, Strand ID: A Detail

Release date

2009-03-04

Citation

Solution NMR structure of ubiquitin-like domain of Arabidopsis thaliana protein At2g32350
Eletsky, A., Mills, J.L., Sukumaran, D., Hua, J., Shastry, R., Jiang, M., Ciccosanti, C., Xiao, R., Liu, J., Everret, J.K., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.
Proteins: Struct. Funct. Genet.

Related entities 1. At2g32350, : 1 : 1 : 1 : 44 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC