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BMRB: 16032

2KAV

Nav1.2 C-terminal EF-Hand Domain

Authors

Miloushev, V.Z., Levine, J.A., Arbing, M.A., Hunt, J.F., Pitt, G.S., Palmer, A.G.

Assembly

NaV1.2 C-terminal EF-hand domain

Entity

1. NaV1.2 C-terminal EF-hand domain (polymer, Thiol state: all free), 129 monomers, 14573.24 Da Detail

MGSSHHHHHH SSGLVPRGSH MASENFSVAT EESAEPLSED DFEMFYEVWE KFDPDATQFI EFAKLSDFAD ALDPPLLIAK PNKVQLIAMD LPMVSGDRIH CLDILFAFTK RVLGESGEMD ALRIQMEER

Formula weight

14573.24 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS19:SG
2	disulfide	sing	1:CYS11:SG	1:CYS24:SG
3	disulfide	sing	1:CYS18:SG	1:CYS31:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, conformer_family_coord_set

Chem. Shift Complete

Sequence coverage: 89.1 %, Completeness: 76.9 %, Completeness (bb): 72.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.9 % (1142 of 1486)	85.6 % (658 of 769)	63.8 % (376 of 589)	84.4 % (108 of 128)
Backbone	72.0 % (547 of 760)	85.2 % (219 of 257)	58.3 % (222 of 381)	86.9 % (106 of 122)
Sidechain	81.6 % (693 of 849)	85.5 % (438 of 512)	76.7 % (254 of 331)	16.7 % (1 of 6)
Aromatic	62.0 % (88 of 142)	74.6 % (53 of 71)	48.6 % (34 of 70)	100.0 % (1 of 1)
Methyl	92.7 % (115 of 124)	93.5 % (58 of 62)	91.9 % (57 of 62)

1. NaV1.2 C-terminal EF-hand domain

MGSSHHHHHH SSGLVPRGSH MASENFSVAT EESAEPLSED DFEMFYEVWE KFDPDATQFI EFAKLSDFAD ALDPPLLIAK PNKVQLIAMD LPMVSGDRIH CLDILFAFTK RVLGESGEMD ALRIQMEER

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 290.5 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 (±0.05) mM
2	glycine	[D5-98%]	100 (±10.0) mM
3	TRIS	[D11-98 %]	20 (±2.0) mM
4	EDTA	[D16-98%]	0.1 (±0.01) mM
5	D2O	[U-99% 2H]	10 (±1.0) %
6	DTT	[D10-98%]	1 (±0.1) mM
7	NaN3	natural abundance	0.02 (±0.002) %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 290.5 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	entity	[U-10% 13C; U-99% 15N]	0.5 (±0.05) mM
9	glycine	[D5-98%]	100 (±10.0) mM
10	TRIS	[D11-98 %]	20 (±2.0) mM
11	EDTA	[D16-98%]	0.1 (±0.01) mM
12	D2O	[U-99% 2H]	10 (±1.0) %
13	DTT	[D10-98%]	1 (±0.1) mM
14	NaN3	natural abundance	0.02 (±0.002) %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 290.5 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Concentration
15	entity	[U-99% 13C; U-99% 15N]	0.5 (±0.05) mM
16	glycine	[D5-98%]	100 (±10.0) mM
17	TRIS	[D11-98 %]	20 (±2.0) mM
18	EDTA	[D16-98%]	0.1 (±0.01) mM
19	D2O	[U-99% 2H]	10 (±1.0) %
20	DTT	[D10-98%]	1 (±0.1) mM
21	NaN3	natural abundance	0.02 (±0.002) %
22	pF1 phage	natural abundance	15 (±1.0) mg

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2KAV, Strand ID: A Detail

Release date

2009-01-19

Citation

Solution structure of the NaV1.2 C-terminal EF-hand domain
Miloushev, V.Z., Levine, J.A., Arbing, M.A., Hunt, J.F., Pitt, G.S., Palmer, A.G.
J. Biol. Chem. (2009), 284, 6446-6454, PubMed 19129176 , DOI 10.1074/jbc.M807401200 , Abstract

Related entities 1. NaV1.2 C-terminal EF-hand domain, : 1 : 1 : 6 : 117 entities Detail

Interaction partners 1. NaV1.2 C-terminal EF-hand domain, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 3D HNCO