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BMRB: 16045

2KBI

1H, 13C, and 15N Chemical Shift Assignments for the C-terminal EF-Hand domain of human cardiac sodium channel NaV1.5

Authors

Chagot, B., Potet, F., Balser, J.R., Chazin, W.J.

Assembly

hH1

Entity

1. hH1 (polymer, Thiol state: all free), 97 monomers, 10987.15 Da Detail

GPGSENFSVA TEESTEPLSE DDFDMFYEIW EKFDPEATQF IEYSVLSDFA DALSEPLRIA KPNQISLINM DLPMVSGDRI HCMDILFAFT KRVLGES

Formula weight

10987.15 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.1 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.1 % (1048 of 1126)	97.8 % (569 of 582)	85.7 % (383 of 447)	99.0 % (96 of 97)
Backbone	98.6 % (562 of 570)	99.0 % (190 of 192)	97.9 % (281 of 287)	100.0 % (91 of 91)
Sidechain	89.2 % (579 of 649)	97.2 % (379 of 390)	77.1 % (195 of 253)	83.3 % (5 of 6)
Aromatic	45.5 % (51 of 112)	91.1 % (51 of 56)	0.0 % (0 of 55)	0.0 % (0 of 1)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. hH1

GPGSENFSVA TEESTEPLSE DDFDMFYEIW EKFDPEATQF IEYSVLSDFA DALSEPLRIA KPNQISLINM DLPMVSGDRI HCMDILFAFT KRVLGES

Show sample condition

Sample #1

Solvent system 100% H2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hH1	[U-100% 13C; U-100% 15N]	1.2 mM
2	H2O	natural abundance	100 %
3	phosphate	natural abundance	100 mM
4	NaCl	natural abundance	200 mM
5	beta-mercaptoethanol	natural abundance	5 mM
6	NaN3	natural abundance	0.01 %

Sample #2

Solvent system 100% H2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	hH1	natural abundance	1.2 mM
8	H2O	natural abundance	100 %
9	phosphate	natural abundance	100 mM
10	NaCl	natural abundance	200 mM
11	beta-mercaptoethanol	natural abundance	5 mM
12	NaN3	natural abundance	0.01 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	methyl protons	4.7 ppm	internal	direct	1.0

Referencing offset: 2.32 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	methyl protons	4.7 ppm	internal	direct	1.0

Referencing offset: 2.32 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	methyl protons	4.7 ppm	internal	direct	1.0

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	methyl protons	4.7 ppm	internal	direct	1.0

Referencing offset: 2.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KBI, Strand ID: A Detail

Release date

2009-02-08

Citation

Solution NMR structure of the C-terminal EF-hand domain of human cardiac sodium channel NaV1.5
Chagot, B., Potet, F., Balser, J.R., Chazin, W.J.
J. Biol. Chem. (2009), 284, 6436-6445, PubMed 19074138 , DOI 10.1074/jbc.M807747200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q14524 released on 1998-12-15
Title SCN5A_HUMAN Entity Sodium channel protein type 5 subunit alpha

Related entities 1. hH1, : 1 : 9 : 117 entities Detail

Interaction partners 1. hH1, : 13 interactors Detail

More details for 13 interactors identified by 9 citations