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BMRB: 16051

2KBN

Solution NMR structure of the OB domain (residues 67-166) of MM0293 from Methanosarcina mazei. Northeast Structural Genomics Consortium target MaR214a.

Authors

Ramelot, T.A., Ding, K., Magliqui, M., Jiang, M., Ciccosanti, C., Xiao, R., Lui, J., Everett, J.K., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T., Kennedy, M.A.

Assembly

MM0293

Entity

1. MM0293 (polymer, Thiol state: not present), 109 monomers, 12502.96 Da Detail

MEPQLTKIVD IVENGQWANL KAKVIQLWEN THESISQVGL LGDETGIIKF TIWKNAELPL LEQGESYLLR SVVVGEYNDR FQVQVNKNSS IEKLSEPIEV GLEHHHHHH

Formula weight

12502.96 Da

Source organism

Methanosarcina mazei

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 92.5 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.5 % (1213 of 1311)	93.0 % (633 of 681)	91.3 % (463 of 507)	95.1 % (117 of 123)
Backbone	93.8 % (608 of 648)	94.6 % (210 of 222)	93.1 % (298 of 320)	94.3 % (100 of 106)
Sidechain	91.6 % (701 of 765)	92.2 % (423 of 459)	90.3 % (261 of 289)	100.0 % (17 of 17)
Aromatic	74.0 % (74 of 100)	74.0 % (37 of 50)	72.3 % (34 of 47)	100.0 % (3 of 3)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. MM0293

MEPQLTKIVD IVENGQWANL KAKVIQLWEN THESISQVGL LGDETGIIKF TIWKNAELPL LEQGESYLLR SVVVGEYNDR FQVQVNKNSS IEKLSEPIEV GLEHHHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; 5%-13C-labeleld

#	Name	Isotope labeling	Concentration
7	OB domain	[U-5% 13C; U-99% 15N]	1.2 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	10 mM
11	calcium chloride	natural abundance	5 mM
12	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KBN, Strand ID: A Detail

Release date

2011-02-23

Citation

NMR structure of the OB domain of mm0293 from the archea methanosarcina mazei
Kennedy, M.A., Ramelot, T.A., Ding, K., Magliqui, M., Jiang, M., Ciccosanti, C., Xiao, R., Lui, J., Everett, J.K., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T.
Not known

Related entities 1. MM0293, : 1 : 1 : 1 entities Detail

Experiments performed 13 experiments Detail

1 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

2 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

3 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

5 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

6 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

7 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

10 2D 1H-15N HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

11 2D 1H-13C HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

12 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; 5%-13C-labeleld

#	Name	Isotope labeling	Concentration
7	OB domain	[U-5% 13C; U-99% 15N]	1.2 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	10 mM
11	calcium chloride	natural abundance	5 mM
12	sodium azide	natural abundance	0.02 %

13 4D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details uniformly 15N-labeled; uniformly-13C-labeleld

#	Name	Isotope labeling	Concentration
1	OB domain	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

nullKeywords NMR structure, OB domain, beta barrel, nucleic acid binding protein

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Found 1 Document (0.899 seconds)

BMRB: 16051

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MM0293, : 1 : 1 : 1 entities Detail

Experiments performed 13 experiments Detail

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