BMRB: 16054

Dimeric solution structure of the DNA loop d(TGCTTCGT)

Authors

Viladoms, J., Escaja, N., Frieden, M., Gomez-Pinto, I., Pedroso, E., Gonzalez, C.

Assembly

5'-D(TP*CP*GP*TP*TP*GP*CP*T)-3'

Entity

1. 5'-D(TP*CP*GP*TP*TP*GP*CP*T)-3' (polymer, Thiol state: not present), 8 monomers, 2471.561 × 2 Da Detail

TGCTTCGT

Total weight

4943.122 Da

Max. entity weight

2471.561 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.7 %, Completeness (bb): 87.5 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	88.7 % (71 of 80)	88.7 % (71 of 80)
Suger, PO4	87.5 % (49 of 56)	87.5 % (49 of 56)
Nucleobase	91.7 % (22 of 24)	91.7 % (22 of 24)
Aromatic	87.5 % (14 of 16)	87.5 % (14 of 16)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. DNA (5'-D(TP*GP*CP*TP*TP*CP*GP*T)-3')

TGCTTCGT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	DNA_(5'-D(TP*GP*CP*TP*TP*CP*GP*T)-3')	natural abundance		0.1 ~ 4.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	magnesium chloride	natural abundance		10 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

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Release date

2009-04-15

Citation

Self-association of short DNA loops through minor groove C:G:G:C tetrads
Viladoms, J., Escaja, N., Frieden, M., Gomez-Pinto, I., Pedroso, E., Gonzalez, C.
Nucleic Acids Res. (2009), 37, 3264-3275, PubMed 19321501 , DOI 10.1093/nar/gkp191 , Abstract

Experiments performed 3 experiments Detail