BMRB: 16055

Dimeric solution structure of the cyclic octamer d(pCGCTCCGT)

Authors

Viladoms, J., Escaja, N., Frieden, M., Gomez-Pinto, I., Pedroso, E., Gonzalez, C.

Assembly

5'-D(P*TP*CP*GP*TP*TP*GP*CP*T)-3'

Entity

1. 5'-D(P*TP*CP*GP*TP*TP*GP*CP*T)-3' (polymer, Thiol state: not present), 8 monomers, 2441.539 × 2 Da Detail

CGCTCCGT

Total weight

4883.078 Da

Max. entity weight

2441.539 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.6 %, Completeness (bb): 87.5 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	86.6 % (71 of 82)	86.6 % (71 of 82)
Suger, PO4	87.5 % (49 of 56)	87.5 % (49 of 56)
Nucleobase	84.6 % (22 of 26)	84.6 % (22 of 26)
Aromatic	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. DNA (cyclic D(P*CP*GP*CP*TP*CP*CP*GP*T))

CGCTCCGT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	DNA (cyclic D(P*CP*GP*CP*TP*CP*CP*GP*T))	natural abundance		0.1 ~ 4.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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Release date

2009-04-16

Citation

Self-association of short DNA loops through minor groove C:G:G:C tetrads
Viladoms, J., Escaja, N., Frieden, M., Gomez-Pinto, I., Pedroso, E., Gonzalez, C.
Nucleic Acids Res. (2009), 37, 3264-3275, PubMed 19321501 , DOI 10.1093/nar/gkp191 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 3 experiments Detail