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BMRB: 16058

2KC0

SOLUTION STRUCTURE OF THE FACTOR H BINDING PROTEIN

Authors

Cantini, F., Veggi, D., Dragonetti, S., Savino, S., Scarselli, M., Romagnoli, G., Pizza, M., Banci, L., Rappuoli, R.

Assembly

Factor H-binding Protein

Entity

1. Factor H-binding Protein (polymer, Thiol state: not present), 255 monomers, 27412.26 Da Detail

MVAADIGAGL ADALTAPLDH KDKGLQSLTL DQSVRKNEKL KLAAQGAEKT YGNGDSLNTG KLKNDKVSRF DFIRQIEVDG QLITLESGEF QVYKQSHSAL TAFQTEQIQD SEHSGKMVAK RQFRIGDIAG EHTSFDKLPE GGRATYRGTA FGSDDAGGKL TYTIDFAAKQ GNGKIEHLKS PELNVDLAAA DIKPDGKRHA VISGSVLYNQ AEKGSYSLGI FGGKAQEVAG SAEVKTVNGI RHIGLAAKQH HHHHH

Formula weight

27412.26 Da

Source organism

Neisseria meningitidis

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 90.6 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.6 % (2588 of 2855)	92.9 % (1375 of 1480)	86.6 % (953 of 1101)	94.9 % (260 of 274)
Backbone	95.5 % (1453 of 1522)	95.7 % (512 of 535)	94.8 % (698 of 736)	96.8 % (243 of 251)
Sidechain	86.7 % (1352 of 1559)	91.3 % (863 of 945)	79.9 % (472 of 591)	73.9 % (17 of 23)
Aromatic	48.9 % (93 of 190)	83.2 % (79 of 95)	14.7 % (14 of 95)
Methyl	94.4 % (255 of 270)	97.0 % (131 of 135)	91.9 % (124 of 135)

1. Factor H-binding Protein

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 (±0.1) mM
2	potassium phosphate	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	entity	[U-98% 13C; U-98% 15N]	0.5 (±0.1) mM
6	potassium phosphate	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	entity	natural abundance	0.8 (±0.1) mM
10	potassium phosphate	natural abundance	50 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methyl carbon	69.4 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.53 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methyl carbon	69.4 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.53 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methyl carbon	69.4 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methyl carbon	69.4 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 1.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2KC0, Strand ID: A Detail

Release date

2009-02-08

Citation

Solution structure of the factor H-binding protein, a survival factor and protective antigen of Neisseria meningitidis
Cantini, F., Veggi, D., Dragonetti, S., Savino, S., Scarselli, M., Romagnoli, G., Pizza, M., Banci, L., Rappuoli, R.
J. Biol. Chem. (2009), 284, 9022-9026, PubMed 19196709 , DOI 10.1074/jbc.C800214200 , Abstract

Related entities 1. Factor H-binding Protein, : 1 : 6 : 20 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC