BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	92.0 % (1103 of 1199)	95.2 % (599 of 629)	87.2 % (410 of 470)	94.0 % (94 of 100)
Backbone	94.0 % (549 of 584)	94.5 % (188 of 199)	93.8 % (271 of 289)	93.8 % (90 of 96)
Sidechain	91.0 % (644 of 708)	95.6 % (411 of 430)	83.6 % (229 of 274)	100.0 % (4 of 4)
Aromatic	43.0 % (37 of 86)	83.7 % (36 of 43)	0.0 % (0 of 42)	100.0 % (1 of 1)
Methyl	100.0 % (122 of 122)	100.0 % (61 of 61)	100.0 % (61 of 61)

1. RelE

GSHMAYFLDF DERALKEWRK LGSTVREQLK KKLVEVLESP RIEANKLRGM PDCYKIKLRS SGYRLVYQVI DEKVVVFVIS VGKAEASEVY SEAVKRIL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296.5 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	RelE	[U-13C; U-15N]	0.5 (±0.1) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	500 mM
4	DTT	natural abundance	1 mM
5	sodium azide	natural abundance	0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 296.5 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Concentration
6	RelE	[U-13C; U-15N]	0.5 (±0.1) mM
7	sodium phosphate	natural abundance	25 mM
8	sodium chloride	natural abundance	500 mM
9	DTT	natural abundance	1 mM
10	sodium azide	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KC9, Strand ID: A Detail

Release date

2009-04-21

Citation

Inhibitory mechanism of Escherichia coli RelE-RelB toxin-antitoxin module involves a helix displacement near an mRNA interferase active site
Li, G., Zhang, Y., Inouye, M., Ikura, M.
J. Biol. Chem. (2009), 284, 14628-14636, PubMed 19297318 , DOI 10.1074/jbc.M809656200 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 2 entries Detail

  BMRB: 16065 released on 2009-04-21
    Title Structure of E. coli toxin RelE(R81A/R83A)mutant in complex with antitoxin RelBc (K47-L79) peptide
  BMRB: 16067 released on 2009-04-21
    Title Backbone assignment of RelB antitoxin C-terminal peptide (RelBc) in the RelE-free state
  Swiss-Prot: P0C077 released on 1988-04-01
    Title RELE_ECOLI Entity mRNA interferase RelE
  Swiss-Prot: P0C079 released on 1988-04-01
    Title RELB_ECOLI Entity Antitoxin RelB

Related entities 1. RelE, : 1 : 1 : 7 : 5 entities Detail

Interaction partners 1. RelE, : 13 interactors Detail

More details for 13 interactors identified by 3 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC