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solution structure of FAPP1 PH domain
Authors
Lenoir, M., Coskun, U., James, J., Simons, K., Overduin, M.
Assembly
FAPP1-PH
Entity
1. FAPP1-PH (polymer, Thiol state: unknown), 108 monomers, 12205.77 Da Detail

GPLGSPEFME GVLYKWTNYL TGWQPRWFVL DNGILSYYDS QDDVCKGSKG SIKMAVCEIK VHSADNTRME LIIPGEQHFY MKAVNAAERQ RWLVALGSSK ASLTDTRT


Formula weight
12205.77 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
molecular dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.1 %, Completeness: 95.9 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All95.9 % (1212 of 1264)96.3 % (631 of 655)94.5 % (466 of 493)99.1 % (115 of 116)
Backbone98.6 % (631 of 640)98.6 % (218 of 221)98.4 % (310 of 315)99.0 % (103 of 104)
Sidechain94.1 % (680 of 723)95.2 % (413 of 434)92.1 % (255 of 277)100.0 % (12 of 12)
Aromatic77.8 % (98 of 126)84.1 % (53 of 63)69.5 % (41 of 59)100.0 % (4 of 4)
Methyl100.0 % (110 of 110)100.0 % (55 of 55)100.0 % (55 of 55)

1. FAPP1-PH

GPLGSPEFME GVLYKWTNYL TGWQPRWFVL DNGILSYYDS QDDVCKGSKG SIKMAVCEIK VHSADNTRME LIIPGEQHFY MKAVNAAERQ RWLVALGSSK ASLTDTRT

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
1FAPP1-PH[U-13C; U-15N]600 uM
2TRISnatural abundance20 mM
3sodium azidenatural abundance1 mM
4sodium chloridenatural abundance100 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
5FAPP1-PH[U-15N]600 uM
6TRISnatural abundance20 mM
7sodium azidenatural abundance1 mM
8sodium chloridenatural abundance100 mM

LACS Plot; CA
Referencing offset: -0.07 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.07 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.06 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2KCJ, Strand ID: A Detail


Release date
2010-01-06
Citation
Molecular basis of phosphatidylinositol 4-phosphate and ARF1 GTPase recognition by the FAPP1 pleckstrin homology (PH) domain
He, J., Scott, J.L., Heroux, A., Roy, S., Lenoir, M., Overduin, M., Stahelin, R.V., Kutateladze, T.G.
J. Biol. Chem. (2011), 286, 18650-18657, PubMed 21454700 , DOI 10.1074/jbc.M111.233015 ,
Entries sharing articles Swiss-Prot: 2 entries Detail
  Swiss-Prot: P84077 released on 2004-08-16
    Title ARF1_HUMAN Entity ADP-ribosylation factor 1
  Swiss-Prot: Q9HB20 released on 2004-03-29
    Title PKHA3_HUMAN Entity Pleckstrin homology domain-containing family A member 3
Related entities 1. FAPP1-PH, : 1 : 7 : 180 entities Detail
Interaction partners 1. FAPP1-PH, : 2 interactors Detail
Experiments performed 9 experiments Detail
NMR combined restraints 5 contents Detail
Keywords FAPP1, NMR, PH domain