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BMRB: 16084

2KCL

SOLUTION STRUCTURE OF TETRATRICOPEPTIDE REPEAT DOMAIN PROTEIN SRU_0103 FROM SALINIBACTER RUBER, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET SrR115C

Authors

Liu, G., Rossi, P., Wang, D., Nwosu, C., Owens, L., Xiao, R., Liu, J., Baran, M.C., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T.

Assembly

SRU 0103

Entity

1. SRU 0103 (polymer, Thiol state: all free), 99 monomers, 11468.26 Da Detail

EDPEDPFTRY ALAQEHLKHD NASRALALFE ELVETDPDYV GTYYHLGKLY ERLDRTDDAI DTYAQGIEVA REEGTQKDLS ELQDAKLKAE GLEHHHHHH

Formula weight

11468.26 Da

Source organism

Salinibacter ruber

Exptl. method

solution NMR

Refine. method

distance geometry, torsion angle dynamics, molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 83.7 %, Completeness (bb): 78.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.7 % (948 of 1133)	92.5 % (542 of 586)	70.0 % (312 of 446)	93.1 % (94 of 101)
Backbone	78.9 % (464 of 588)	93.5 % (187 of 200)	64.0 % (187 of 292)	93.8 % (90 of 96)
Sidechain	89.0 % (569 of 639)	92.0 % (355 of 386)	84.7 % (210 of 248)	80.0 % (4 of 5)
Aromatic	61.5 % (64 of 104)	75.0 % (39 of 52)	48.1 % (25 of 52)
Methyl	94.0 % (94 of 100)	94.0 % (47 of 50)	94.0 % (47 of 50)

1. SRU 0103

EDPEDPFTRY ALAQEHLKHD NASRALALFE ELVETDPDYV GTYYHLGKLY ERLDRTDDAI DTYAQGIEVA REEGTQKDLS ELQDAKLKAE GLEHHHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SRU_0103	[U-100% 13C; U-100% 15N]		1.37 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
2	SRU_0103	[U-10% 13C; U-100% 15N]		1.05 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KCL, Strand ID: A Detail

Release date

2009-01-08

Citation

SOLUTION STRUCTURE OF TETRATRICOPEPTIDE REPEAT DOMAIN PROTEIN SRU_0103 FROM SALINIBACTER RUBER, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET SrR115C
Liu, G., Rossi, P., Wang, D., Nwosu, C., Owens, L., Xiao, R., Liu, J., Baran, M.C., Swapna, G., Acton, T.B., Rost, B., Montelione, G.T.
Not known

Related entities 1. SRU 0103, : 1 : 2 : 30 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC