BMRB: 16085

1H, 13C, 15N Chemical Shift Assignment for Saccharomyces cerevisiae Acyl Carrier Protein.

Authors

Perez, D.R.

Assembly

ACP single polypeptide chain

Entity

1. ACP single polypeptide chain (polymer, Thiol state: not present), 183 monomers, 19792.24 Da Detail

MGSSHHHHHH ENLYFQSNAE IADEPVKASL LLHVLVAHKL KKSLDSIPMS KTIKDLVGGK STVQNEILGD LGKEFGTTPE KPEETPLEEL AETFQDTFSG ALGKQSSSLL SRLISSKMPG GFTITVARKY LQTRWGLPSG RQDGVLLVAL SNEPAARLGS EADAKAFLDS MAQKYASIVG VDL

Formula weight

19792.24 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 91.1 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.1 % (1893 of 2079)	91.0 % (979 of 1076)	90.7 % (740 of 816)	93.0 % (174 of 187)
Backbone	91.6 % (991 of 1082)	91.7 % (342 of 373)	90.8 % (485 of 534)	93.7 % (164 of 175)
Sidechain	90.7 % (1057 of 1165)	90.6 % (637 of 703)	91.1 % (410 of 450)	83.3 % (10 of 12)
Aromatic	67.2 % (86 of 128)	67.2 % (43 of 64)	66.7 % (42 of 63)	100.0 % (1 of 1)
Methyl	97.7 % (209 of 214)	97.2 % (104 of 107)	98.1 % (105 of 107)

1. Acyl Carrier Protein

MGSSHHHHHH ENLYFQSNAE IADEPVKASL LLHVLVAHKL KKSLDSIPMS KTIKDLVGGK STVQNEILGD LGKEFGTTPE KPEETPLEEL AETFQDTFSG ALGKQSSSLL SRLISSKMPG GFTITVARKY LQTRWGLPSG RQDGVLLVAL SNEPAARLGS EADAKAFLDS MAQKYASIVG VDL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Acyl Carrier Protein	[U-13C; U-15N]		1 mM
2	KH2PO4 : K2HPO4 (0.685 : 0.315) buffer	natural abundance		52 mM

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LACS Plot; CA

Referencing offset: 0.1 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.1 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.68 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ML8, Strand ID: A Detail

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Release date

2009-04-15

Citation

1H, 15N, 13C resonance assignment of the acyl carrier protein subunit of the Saccharomyces cerevisiae fatty acid synthase
Perez, D.R., Wider, G.
Biomol. NMR Assign. (2009), 3, 133-136, PubMed 19636964 , DOI 10.1007/s12104-009-9158-4 , Abstract

Related entities 1. ACP single polypeptide chain, : 1 : 13 : 13 entities Detail

Interaction partners 1. ACP single polypeptide chain, : 38 interactors Detail

Experiments performed 5 experiments Detail