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BMRB: 16089

2KCO

Solution NMR structure of ribosomal protein sso0164 from Sulfolobus solfataricus. NortheastStructural Genomics Consortium (NESG) target SsT4

Authors

Lemak, A., Gutmanas, A., Yee, A., Fares, C., Garcia, M., Montelione, G., Arrowsmith, C.

Assembly

sso0164

Entity

1. sso0164 (polymer, Thiol state: not present), 133 monomers, 14658.74 Da Detail

MGFYQGPDNR KITGGLKGKH RDKRKYEIGN PPTFTTLSAE DIRIKDRTLG GNFKVRLKYT TTANVLDPAT NTAKKVKILE ILETPANKEL ARRGIIIRGA KIRTEAGLAV VTSRPGQDGV INAVLLKNES QRS

Formula weight

14658.74 Da

Source organism

Saccharolobus solfataricus

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics in water bath

Data set

RDCs, assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 83.8 %, Completeness (bb): 86.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.8 % (1303 of 1554)	84.0 % (687 of 818)	83.3 % (498 of 598)	85.5 % (118 of 138)
Backbone	86.9 % (683 of 786)	88.3 % (241 of 273)	86.8 % (335 of 386)	84.3 % (107 of 127)
Sidechain	81.5 % (724 of 888)	81.5 % (444 of 545)	81.3 % (270 of 332)	90.9 % (10 of 11)
Aromatic	55.2 % (32 of 58)	72.4 % (21 of 29)	37.9 % (11 of 29)
Methyl	91.4 % (148 of 162)	91.4 % (74 of 81)	91.4 % (74 of 81)

1. sso0164

MGFYQGPDNR KITGGLKGKH RDKRKYEIGN PPTFTTLSAE DIRIKDRTLG GNFKVRLKYT TTANVLDPAT NTAKKVKILE ILETPANKEL ARRGIIIRGA KIRTEAGLAV VTSRPGQDGV INAVLLKNES QRS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 7.7

#	Name	Isotope labeling	Concentration
1	sso0164	[U-13C; U-15N]	0.5 mM
2	TRIS	natural abundance	10 mM
3	sodium chloride	natural abundance	500 mM
4	ZnSO4	natural abundance	10 uM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KCO, Strand ID: A Detail

RDC

30 RDC values in 1 lists
Field strength (¹H) 800 MHz, Pressure 1.0 atm, Temperature 298 K, pH 7.7 Detail

Release date

2010-09-23

Citation

Solution NMR Structure of ribosomal protein sso0164 from Sulfolobus solfataricus
Lemak, A., Gutmanas, A., Yee, A., Fares, C., Garcia, M., Montelione, G., Arrowsmith, C.
Not known

Related entities 1. sso0164, : 1 : 1 : 96 entities Detail

Experiments performed 12 experiments Detail

1 3D HNCO