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BMRB: 16099

2KCX

Solution NMR Structure of KAZAL-1 domain from Human Follistatin-related protein 3 (FSTL-3). Northeast Structural Genomics Target HR6186A.

Authors

Rossi, P., Chiang, Y., Montelione, G.T., Anderson, S.

Assembly

FSTL-3

Entity

1. FSTL-3 (polymer, Thiol state: all disulfide bound), 74 monomers, 7967.047 Da Detail

SDSCDGVECG PGKACRMLGG RPRCECAPDC SGLPARLQVC GSDGATYRDE CELRAARCRG HPDLSVMYRG RCRK

Formula weight

7967.047 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 93.9 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (722 of 769)	92.4 % (377 of 408)	95.5 % (278 of 291)	95.7 % (67 of 70)
Backbone	95.6 % (415 of 434)	94.8 % (145 of 153)	96.2 % (204 of 212)	95.7 % (66 of 69)
Sidechain	92.5 % (369 of 399)	91.0 % (232 of 255)	95.1 % (136 of 143)	100.0 % (1 of 1)
Aromatic	60.0 % (12 of 20)	60.0 % (6 of 10)	60.0 % (6 of 10)
Methyl	93.5 % (43 of 46)	91.3 % (21 of 23)	95.7 % (22 of 23)

1. FSTL-3

SDSCDGVECG PGKACRMLGG RPRCECAPDC SGLPARLQVC GSDGATYRDE CELRAARCRG HPDLSVMYRG RCRK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	FSTL-3	[U-100% 13C; U-100% 15N]	0.6 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	FSTL-3	[U-100% 15N]	0.2 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	200 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KCX, Strand ID: A Detail

Release date

2010-09-16

Citation

Solution NMR Structure of Kazal-1 Domain of Human Follistatin-related protein 3 (FSTL-3). Northeast Structural Genomics Target HR6186A
Rossi, P., Chiang, Y., Montelione, G.T., Anderson, S.
Not known

Related entities 1. FSTL-3, : 1 : 4 : 182 entities Detail

Interaction partners 1. FSTL-3, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC