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Solution structure of the conserved C-terminal dimerization domain of Borealin
Authors
Lingel, A., Fairbrother, W.
Assembly
Borealin
Entity
1. Borealin (polymer, Thiol state: all free), 76 monomers, 8071.066 × 2 Da Detail

GSAGERIYNI SGNGSPLADS KEIFLTVPVG GGESLRLLAS DLQRHSIAQL DPEALGNIKK LSNRLAQICS SIRTHK


Total weight
16142.132 Da
Max. entity weight
8071.066 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.4 %, Completeness: 80.5 %, Completeness (bb): 75.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All80.5 % (682 of 847)85.3 % (377 of 442)72.3 % (235 of 325)87.5 % (70 of 80)
Backbone75.1 % (338 of 450)85.4 % (134 of 157)63.2 % (139 of 220)89.0 % (65 of 73)
Sidechain88.0 % (409 of 465)85.3 % (243 of 285)93.1 % (161 of 173)71.4 % (5 of 7)
Aromatic84.6 % (22 of 26)100.0 % (13 of 13)69.2 % (9 of 13)
Methyl95.7 % (88 of 92)95.7 % (44 of 46)95.7 % (44 of 46)

1. Borealin

GSAGERIYNI SGNGSPLADS KEIFLTVPVG GGESLRLLAS DLQRHSIAQL DPEALGNIKK LSNRLAQICS SIRTHK

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2


#NameIsotope labelingTypeConcentration
1Borealinnatural abundance0.6 mM
2sodium phosphatenatural abundance40 mM
3sodium chloridenatural abundance100 mM
4D2Onatural abundance100 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2


#NameIsotope labelingTypeConcentration
5Borealin[U-100% 15N]0.8 mM
6sodium phosphatenatural abundance40 mM
7sodium chloridenatural abundance100 mM
8H2Onatural abundance95 %
9D2Onatural abundance5 %
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2


#NameIsotope labelingTypeConcentration
10Borealin[U-100% 13C; U-100% 15N]0.7 mM
11sodium phosphatenatural abundance40 mM
12sodium chloridenatural abundance100 mM
13H2Onatural abundance95 %
14D2Onatural abundance5 %
Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2


#NameIsotope labelingTypeConcentration
15Borealin[U-100% 13C; U-100% 15N]0.7 mM
16sodium phosphatenatural abundance40 mM
17sodium chloridenatural abundance100 mM
18D2Onatural abundance100 %
Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2


#NameIsotope labelingTypeConcentration
19Borealin[U-100% 13C; U-100% 15N]0.4 mM
20Borealinnatural abundance0.4 mM
21sodium phosphatenatural abundance40 mM
22sodium chloridenatural abundance100 mM
23D2Onatural abundance100 %

LACS Plot; CA
Referencing offset: 0.61 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: 0.61 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 2KDD, Strand ID: A, B Detail


Release date
2009-06-24
Citation
Phosphorylation of a borealin dimerization domain is required for proper chromosome segregation
Bourhis, E., Lingel, A., Phung, Q., Fairbrother, W.J., Cochran, A.G.
Biochemistry (2009), 48, 6783-6793, PubMed 19530738 , DOI 10.1021/bi900530v ,
Related entities 1. Borealin, : 1 : 12 entities Detail
Experiments performed 14 experiments Detail
NMR combined restraints 5 contents Detail
Keywords protein dimer