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BMRB: 16110

2KDD

Solution structure of the conserved C-terminal dimerization domain of Borealin

Authors

Lingel, A., Fairbrother, W.

Assembly

Borealin

Entity

1. Borealin (polymer, Thiol state: all free), 76 monomers, 8071.066 × 2 Da Detail

GSAGERIYNI SGNGSPLADS KEIFLTVPVG GGESLRLLAS DLQRHSIAQL DPEALGNIKK LSNRLAQICS SIRTHK

Total weight

16142.132 Da

Max. entity weight

8071.066 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.4 %, Completeness: 80.5 %, Completeness (bb): 75.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.5 % (682 of 847)	85.3 % (377 of 442)	72.3 % (235 of 325)	87.5 % (70 of 80)
Backbone	75.1 % (338 of 450)	85.4 % (134 of 157)	63.2 % (139 of 220)	89.0 % (65 of 73)
Sidechain	88.0 % (409 of 465)	85.3 % (243 of 285)	93.1 % (161 of 173)	71.4 % (5 of 7)
Aromatic	84.6 % (22 of 26)	100.0 % (13 of 13)	69.2 % (9 of 13)
Methyl	95.7 % (88 of 92)	95.7 % (44 of 46)	95.7 % (44 of 46)

1. Borealin

GSAGERIYNI SGNGSPLADS KEIFLTVPVG GGESLRLLAS DLQRHSIAQL DPEALGNIKK LSNRLAQICS SIRTHK

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Borealin	natural abundance	0.6 mM
2	sodium phosphate	natural abundance	40 mM
3	sodium chloride	natural abundance	100 mM
4	D2O	natural abundance	100 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2

#	Name	Isotope labeling	Concentration
5	Borealin	[U-100% 15N]	0.8 mM
6	sodium phosphate	natural abundance	40 mM
7	sodium chloride	natural abundance	100 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2

#	Name	Isotope labeling	Concentration
10	Borealin	[U-100% 13C; U-100% 15N]	0.7 mM
11	sodium phosphate	natural abundance	40 mM
12	sodium chloride	natural abundance	100 mM
13	H2O	natural abundance	95 %
14	D2O	natural abundance	5 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2

#	Name	Isotope labeling	Concentration
15	Borealin	[U-100% 13C; U-100% 15N]	0.7 mM
16	sodium phosphate	natural abundance	40 mM
17	sodium chloride	natural abundance	100 mM
18	D2O	natural abundance	100 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 299 K, pH 7.2

#	Name	Isotope labeling	Concentration
19	Borealin	[U-100% 13C; U-100% 15N]	0.4 mM
20	Borealin	natural abundance	0.4 mM
21	sodium phosphate	natural abundance	40 mM
22	sodium chloride	natural abundance	100 mM
23	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KDD, Strand ID: A, B Detail

Release date

2009-06-24

Citation

Phosphorylation of a borealin dimerization domain is required for proper chromosome segregation
Bourhis, E., Lingel, A., Phung, Q., Fairbrother, W.J., Cochran, A.G.
Biochemistry (2009), 48, 6783-6793, PubMed 19530738 , DOI 10.1021/bi900530v , Abstract