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BMRB: 16111

2KPY

Sequence-specific 1H, 15N and 13C resonance assignments of Art v 1: a proline-rich allergen of Artemisia vulgaris pollen

Authors

Razzera, G., Gadermaier, G., Almeida, M., Ferreira, F., Almeida, F., Valente, A.

Assembly

Art v 1- the major allergen of Artemisia vulgaris

Entity

1. Art v 1- the major allergen of Artemisia vulgaris (polymer, Thiol state: all disulfide bound), 108 monomers, 10799.91 Da Detail

AGSKLCEKTS KTYSGKCDNK KCDKKCIEWE KAQHGACHKR EAGKESCFCY FDCSKSPPGA TPAPPGAAPP PAAGGSPSPP ADGGSPPPPA DGGSPPVDGG SPPPPSTH

Formula weight

10799.91 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS53:SG
2	disulfide	sing	1:CYS17:SG	1:CYS37:SG
3	disulfide	sing	1:CYS22:SG	1:CYS47:SG
4	disulfide	sing	1:CYS26:SG	1:CYS49:SG

Source organism

Artemisia vulgaris

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.6 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.6 % (975 of 1126)	95.2 % (570 of 599)	72.5 % (317 of 437)	97.8 % (88 of 90)
Backbone	84.0 % (509 of 606)	99.0 % (207 of 209)	70.0 % (217 of 310)	97.7 % (85 of 87)
Sidechain	90.9 % (558 of 614)	93.1 % (363 of 390)	86.9 % (192 of 221)	100.0 % (3 of 3)
Aromatic	60.0 % (36 of 60)	83.3 % (25 of 30)	34.5 % (10 of 29)	100.0 % (1 of 1)
Methyl	95.5 % (42 of 44)	95.5 % (21 of 22)	95.5 % (21 of 22)

1. Art v 1

AGSKLCEKTS KTYSGKCDNK KCDKKCIEWE KAQHGACHKR EAGKESCFCY FDCSKSPPGA TPAPPGAAPP PAAGGSPSPP ADGGSPPPPA DGGSPPVDGG SPPPPSTH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.3

#	Name	Isotope labeling	Type	Concentration
1	Art v 1	[U-99% 13C; U-99% 15N]		1 mM
2	Potassium Acetate	natural abundance		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KPY, Strand ID: A Detail

Release date

2009-03-16

Citation

Sequence-specific 1H, 15N and 13C resonance assignments of Art v 1: a proline-rich allergen of Artemisia vulgaris pollen
Razzera, G., Gadermaier, G., Almeida, M.S., Ferreira, F., Almeida, F.C.L., Valente, A.
Biomol. NMR Assign. (2009), 3, 103-106, PubMed 19636957 , DOI 10.1007/s12104-009-9151-y , Abstract

Related entities 1. Art v 1- the major allergen of Artemisia vulgaris, : 1 : 1 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC