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BMRB: 16126

2KDX

Solution structure of HypA protein

Authors

Xia, W., Li, H., Sze, K.

Assembly

HypA

Entity

1. HypA (polymer, Thiol state: free and other bound), 119 monomers, 13346.22 Da Detail

GSMHEYSVVS SLIALCEEHA KKNQAHKIER VVVGIGERSA MDKSLFVSAF ETFREESLVC KDAILDIVDE KVELECKDCS HVFKPNALDY GVCEKCHSKN VIITQGNEMR LLSLEMLAE

2. ZN (non-polymer), 65.409 Da

Total weight

13411.629 Da

Max. entity weight

13346.22 Da

Source organism

Helicobacter pylori

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 93.6 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.6 % (1280 of 1367)	93.9 % (665 of 708)	93.1 % (498 of 535)	94.4 % (117 of 124)
Backbone	96.1 % (684 of 712)	94.2 % (228 of 242)	97.7 % (344 of 352)	94.9 % (112 of 118)
Sidechain	92.1 % (708 of 769)	93.8 % (437 of 466)	89.6 % (266 of 297)	83.3 % (5 of 6)
Aromatic	67.1 % (51 of 76)	84.2 % (32 of 38)	50.0 % (19 of 38)
Methyl	90.7 % (127 of 140)	91.4 % (64 of 70)	90.0 % (63 of 70)

1. HypA

GSMHEYSVVS SLIALCEEHA KKNQAHKIER VVVGIGERSA MDKSLFVSAF ETFREESLVC KDAILDIVDE KVELECKDCS HVFKPNALDY GVCEKCHSKN VIITQGNEMR LLSLEMLAE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	HypA	[U-99% 13C; U-99% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	-0.121 ppm	internal	indirect	0.251
¹H	DSS	methyl protons	-0.121 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	-0.121 ppm	internal	indirect	0.101

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	-0.121 ppm	internal	indirect	0.251
¹H	DSS	methyl protons	-0.121 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	-0.121 ppm	internal	indirect	0.101

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	-0.121 ppm	internal	indirect	0.251
¹H	DSS	methyl protons	-0.121 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	-0.121 ppm	internal	indirect	0.101

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	-0.121 ppm	internal	indirect	0.251
¹H	DSS	methyl protons	-0.121 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	-0.121 ppm	internal	indirect	0.101

Referencing offset: 0.81 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KDX, Strand ID: A Detail

Release date

2009-07-07

Citation

Structure of a nickel chaperone, HypA, from Helicobacter pylori reveals two distinct metal binding sites
Xia, W., Li, H., Sze, K., Sun, H.
J. Am. Chem. Soc. (2009), 131, 10031-10040, PubMed 19621959 , DOI 10.1021/ja900543y , Abstract

Related entities 1. HypA, : 1 : 2 : 78 entities Detail

Interaction partners 1. HypA, : 19 interactors Detail

More details for 19 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC