BMRB: 16138

NMR solution structure of metal-modified DNA

Authors

Johannsen, S., Duepre, N., Boehme, D., Mueller, J., Sigel, R.K.O.

Assembly

34-MER, SILVER ION

Entity

1. DNA (34-MER) (polymer, Thiol state: not present), 34 monomers, 10138.55 Da Detail

TTAATTTXXX AAATTAATTA ATTTXXXAAA TTAA

2. AG (non-polymer), 107.868 × 3 Da

Total weight

10462.154 Da

Max. entity weight

10138.55 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.4 %, Completeness: 93.2 %, Completeness (bb): 98.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polydeoxyribonucleotide

	Total	1H
All	93.2 % (248 of 266)	93.2 % (248 of 266)
Suger, PO4	98.0 % (192 of 196)	98.0 % (192 of 196)
Nucleobase	80.0 % (56 of 70)	80.0 % (56 of 70)
Aromatic	75.0 % (42 of 56)	75.0 % (42 of 56)
Methyl	100.0 % (14 of 14)	100.0 % (14 of 14)

1. DNA (34-MER)

TTAATTTXXX AAATTAATTA ATTTXXXAAA TTAA

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	DNA (34-MER)	natural abundance		0.3 ~ 0.5 mM
2	sodium perchlorate	natural abundance		120 mM
3	SILVER ION	natural abundance		0.9 ~ 1.5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
4	DNA (34-MER)	natural abundance		0.3 ~ 0.5 mM
5	sodium perchlorate	natural abundance		120 mM
6	SILVER ION	natural abundance		0.9 ~ 1.5 mM

Hide sample codition

Release date

2012-08-06

Citation

Solution structure of a DNA double helix with consecutive metal-mediated base pairs
Johannsen, S., Megger, N., Boehme, D., Sigel, R.K.O., Mueller, J.
Nat. Chem. (2010), 2, 229-234, PubMed 21124482 , DOI 10.1038/nchem.512 , Abstract

Experiments performed 6 experiments Detail