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BMRB: 16155

2KEO

Solution NMR structure of protein HS00059 from Homo Sapiens. Northeast Structural Genomics Consortium (NESG) target HT98A.

Authors

Lemak, A., Gutmanas, A., Fares, C., Quyang, H., Dhe-Paganon, S., Montelione, G., Arrowsmith, C.

Assembly

HS00059

Entity

1. HS00059 (polymer, Thiol state: not present), 112 monomers, 12636.67 Da Detail

MHHHHHHSSG RENLYFQGNN EKVTLVRIAD LENHNNDGGF WTVIDGKVYD IKDFQTQSLT ENSILAQFAG EDPVVALEAA LQFEDTRESM HAFCVGQYLE PDQEGVTIPD LG

Formula weight

12636.67 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics in water bath

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 84.8 %, Completeness: 79.3 %, Completeness (bb): 82.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.3 % (1012 of 1276)	79.7 % (523 of 656)	78.4 % (389 of 496)	80.6 % (100 of 124)
Backbone	82.1 % (547 of 666)	81.3 % (187 of 230)	82.9 % (271 of 327)	81.7 % (89 of 109)
Sidechain	77.4 % (552 of 713)	78.9 % (336 of 426)	75.4 % (205 of 272)	73.3 % (11 of 15)
Aromatic	46.9 % (60 of 128)	57.8 % (37 of 64)	34.9 % (22 of 63)	100.0 % (1 of 1)
Methyl	96.5 % (110 of 114)	96.5 % (55 of 57)	96.5 % (55 of 57)

1. hs00059

MHHHHHHSSG RENLYFQGNN EKVTLVRIAD LENHNNDGGF WTVIDGKVYD IKDFQTQSLT ENSILAQFAG EDPVVALEAA LQFEDTRESM HAFCVGQYLE PDQEGVTIPD LG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	hs00059	[U-13C; U-15N]	0.5 mM
2	TRIS	natural abundance	10 mM
3	sodium chloride	natural abundance	300 mM
4	ZnSO4	natural abundance	10 uM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KEO, Strand ID: A Detail

Release date

2009-03-11

Citation

Solution NMR Structure of human protein HS00059
Lemak, A., Gutmanas, A., Fares, C., Quyang, H., Dhe-Paganon, S., Montelione, G., Arrowsmith, C.
Not known

Related entities 1. HS00059, : 1 : 27 entities Detail

Interaction partners 1. HS00059, : 52 interactors Detail

More details for 52 interactors identified by 18 citations

Experiments performed 9 experiments Detail

1 3D HNCO