BMRB: 16168

STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD1 IN MICELLES

Authors

Teriete, P., Franzin, C.M., Choi, J., Marassi, F.M.

Assembly

PHOSPHOLEMMAN

Entity

1. PHOSPHOLEMMAN (polymer, Thiol state: all free), 72 monomers, 8383.458 Da Detail

ESPKEHDPFT YDYQSLQIGG LVIAGILFIL GILIVLSRRC RCKFNQQQRT GEPDEEEGTF RSSIRRLSTR RR

Formula weight

8383.458 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 69.8 %, Completeness (bb): 81.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	69.8 % (606 of 868)	84.8 % (390 of 460)	44.1 % (147 of 333)	92.0 % (69 of 75)
Backbone	81.7 % (348 of 426)	91.8 % (135 of 147)	69.0 % (145 of 210)	98.6 % (68 of 69)
Sidechain	63.8 % (324 of 508)	81.5 % (255 of 313)	36.0 % (68 of 189)	16.7 % (1 of 6)
Aromatic	21.7 % (13 of 60)	43.3 % (13 of 30)	0.0 % (0 of 30)
Methyl	47.3 % (35 of 74)	91.9 % (34 of 37)	2.7 % (1 of 37)

1. FXYD1

ESPKEHDPFT YDYQSLQIGG LVIAGILFIL GILIVLSRRC RCKFNQQQRT GEPDEEEGTF RSSIRRLSTR RR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313.15 K, pH 5.0, Details 1 MM [U-100% 15N] PROTEIN, 20 MM SODIUM CITRATE AT PH 5, 10 MM DTT, 500 MM SDS, 90% H2O/ 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	FXYD1	[U-100% 13C; U-100% 15N]		0.5 mM
2	sodium citrate	natural abundance		20 mM
3	DTT	natural abundance		10 mM
4	SDS	natural abundance		500 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313.15 K, pH 5.0, Details 1 MM [U-100% 15N] PROTEIN, 20 MM SODIUM CITRATE AT PH 5, 10 MM DTT, 500 MM SDS, 90% H2O/ 10% D2O

#	Name	Isotope labeling	Type	Concentration
7	FXYD1	[U-100% 15N]		1 mM
8	sodium citrate	natural abundance		20 mM
9	DTT	natural abundance		10 mM
10	SDS	natural abundance		500 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %

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Calculated from 20 models in PDB: 2JO1, Strand ID: A Detail

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Release date

2009-03-05

Citation

Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles
Teriete, P., Franzin, C.M., Choi, J., Marassi, F.M.
Biochemistry (2007), 46, 6774-6783, PubMed 17511473 , DOI 10.1021/bi700391b , Abstract

Related entities 1. PHOSPHOLEMMAN, : 1 : 2 : 2 : 23 entities Detail

Experiments performed 4 experiments Detail