BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	84.1 % (726 of 863)	91.1 % (418 of 459)	73.7 % (244 of 331)	87.7 % (64 of 73)
Backbone	80.9 % (330 of 408)	96.4 % (135 of 140)	65.3 % (132 of 202)	95.5 % (63 of 66)
Sidechain	87.1 % (452 of 519)	86.8 % (277 of 319)	90.2 % (174 of 193)	14.3 % (1 of 7)
Aromatic	78.0 % (64 of 82)	95.1 % (39 of 41)	64.1 % (25 of 39)	0.0 % (0 of 2)
Methyl	93.1 % (54 of 58)	93.1 % (27 of 29)	93.1 % (27 of 29)

1. yeast Prp40 FF4 domain

GAMGNERRIL EQKKHYFWLL LQRTYTKTGK PKPSTWDLAS KELGESLEYK ALGDEDNIRR QIFEDFKPE

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	yeast Prp40 FF4 domain	natural abundance	0.5 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	130 mM
4	sodium azide	natural abundance	0.5 mM
5	H20	natural abundance	90 %
6	D20	natural abundance	10 %

Solvent system 100% D2O, Pressure 1.0 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Concentration
7	yeast Prp40 FF4 domain	[U-100% 15N]	0.5 mM
8	yeast Prp40 FF4 domain	[U-100% 13C]	0.5 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	130 mM
11	sodium azide	natural abundance	0.5 mM
12	D20	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.53 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.53 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2KFD, Strand ID: A Detail

Release date

2009-08-09

Citation

Solution structure of the fourth FF domain of yeast Prp40 splicing factor
Bonet, R., Ruiz, L., Morales, B., Macias, M.J.
Proteins (2009), 77, 1000-1003, PubMed 19722265 , DOI 10.1002/prot.22547 , Abstract

1 2D 1H-1H NOESY

Bruker DRX - 600 MHz

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	yeast Prp40 FF4 domain	natural abundance	0.5 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	130 mM
4	sodium azide	natural abundance	0.5 mM
5	H20	natural abundance	90 %
6	D20	natural abundance	10 %

2 2D 1H-1H TOCSY

Bruker DRX - 600 MHz

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	yeast Prp40 FF4 domain	natural abundance	0.5 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	130 mM
4	sodium azide	natural abundance	0.5 mM
5	H20	natural abundance	90 %
6	D20	natural abundance	10 %

3 3D CBCA(CO)NH