Structure of the C-terminal domain of EHD1 with FNYESTNPFTAK
GPLGSDDVEW VVGKDKPTYD EIFYTLSPVN GKITGANAKK EMVKSKLPNT VLGKIWKLAD VDKDGLLDDE EFALANHLIK VKLEGHELPA DLPPHLVPPS KRRHE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.5 % (1126 of 1382) | 91.4 % (660 of 722) | 68.8 % (375 of 545) | 79.1 % (91 of 115) |
Backbone | 72.4 % (494 of 682) | 88.8 % (206 of 232) | 58.9 % (202 of 343) | 80.4 % (86 of 107) |
Sidechain | 89.6 % (725 of 809) | 92.7 % (454 of 490) | 85.5 % (266 of 311) | 62.5 % (5 of 8) |
Aromatic | 76.9 % (80 of 104) | 86.5 % (45 of 52) | 68.0 % (34 of 50) | 50.0 % (1 of 2) |
Methyl | 94.6 % (123 of 130) | 96.9 % (63 of 65) | 92.3 % (60 of 65) |
1. EH domain of EHD1
GPLGSDDVEW VVGKDKPTYD EIFYTLSPVN GKITGANAKK EMVKSKLPNT VLGKIWKLAD VDKDGLLDDE EFALANHLIK VKLEGHELPA DLPPHLVPPS KRRHE2. Rab11-FIP2 NPF peptide
FNYESTNPFT AKSolvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 7.0, Details 20mM Tris, 100Kcl, 2mM Cacl2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | EH domain of EHD1 | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | Rab11-FIP2 NPF peptide | natural abundance | 3 mM | |
3 | H20 | natural abundance | 90 % | |
4 | D20 | natural abundance | 10 % |