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BMRB: 16183

2KFL

Tammar Wallaby Prion Protein (121-230)

Authors

Christen, B., Hornemann, S., Damberger, F.F., Wuthrich, K.

Assembly

Tammar Wallaby Prion Protein (121-230)

Entity

1. Tammar Wallaby Prion Protein (121-230) (polymer, Thiol state: all disulfide bound), 112 monomers, 13136.43 Da Detail

GSVVGGLGGY MLGSAMSRPV MHFGNEYEDR YYRENQYRYP NQVMYRPIDQ YGSQNSFVHD CVNITVKQHT TTTTTKGENF TETDIKIMER VVEQMCITQY QNEYQAAQRY YN

Formula weight

13136.43 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Notamacropus eugenii

Exptl. method

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.2 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.2 % (1281 of 1318)	99.1 % (681 of 687)	94.2 % (475 of 504)	98.4 % (125 of 127)
Backbone	98.6 % (657 of 666)	97.4 % (224 of 230)	99.7 % (326 of 327)	98.2 % (107 of 109)
Sidechain	96.3 % (727 of 755)	100.0 % (457 of 457)	90.0 % (252 of 280)	100.0 % (18 of 18)
Aromatic	79.7 % (110 of 138)	100.0 % (69 of 69)	59.4 % (41 of 69)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. wpp 121-230

GSVVGGLGGY MLGSAMSRPV MHFGNEYEDR YYRENQYRYP NQVMYRPIDQ YGSQNSFVHD CVNITVKQHT TTTTTKGENF TETDIKIMER VVEQMCITQY QNEYQAAQRY YN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

LACS Plot; CA

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: 2.34 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KFL, Strand ID: A Detail

Release date

2009-05-19

Citation

Prion protein NMR structure from tammar wallaby (Macropus eugenii) shows that the beta2-alpha2 loop is modulated by long-range sequence effects
Christen, B., Hornemann, S., Damberger, F.F., Wuthrich, K.
J. Mol. Biol. (2009), 389, 833-845, PubMed 19393664 , DOI 10.1016/j.jmb.2009.04.040 , Abstract

Related entities 1. Tammar Wallaby Prion Protein (121-230), : 1 : 154 entities Detail

Experiments performed 10 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

2 3D 1H-13C NOESY

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

3 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

4 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

5 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

7 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

8 3D HCCH-COSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

9 3D H(CCCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

10 3D (H)C(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.1 K, pH 4.5, Details The sample additionally contains a protease inhibitor cocktail.

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-100% 2H]		10 mM
2	entity	[U-99% 13C; U-99% 15N]		1.5 mM
3	sodium azide	natural abundance		0.02 %

nullKeywords Tammar Wallaby PrP