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BMRB: 16191

2KLE

NMR-Assignments of a Cytosolic Domain of the C-Terminus of Polycystin-2

Authors

Kalbitzer, H.

Assembly

Monomer

Entity

1. Polycystin-2 Polypeptide (polymer, Thiol state: not present), 123 monomers, 14001.23 Da Detail

GSTAIGINDT YSEVKSDLAQ QKAEMELSDL IRKGYHKALV KLKLKKNTVD DISESLRQGG GKLNFDELRQ DLKGKGHTDA EIEAIFTKYD QDGDQELTEH EHQQMRDDLE KEREDLDLDH SSL

2. CA (non-polymer), 40.078 Da

Total weight

14041.309 Da

Max. entity weight

14001.23 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.9 %, Completeness: 70.2 %, Completeness (bb): 76.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.2 % (999 of 1423)	70.1 % (524 of 748)	68.2 % (369 of 541)	79.1 % (106 of 134)
Backbone	76.2 % (562 of 738)	77.3 % (197 of 255)	73.9 % (266 of 360)	80.5 % (99 of 123)
Sidechain	65.2 % (521 of 799)	66.3 % (327 of 493)	63.4 % (187 of 295)	63.6 % (7 of 11)
Aromatic	81.3 % (52 of 64)	81.3 % (26 of 32)	81.3 % (26 of 32)
Methyl	60.0 % (72 of 120)	61.7 % (37 of 60)	58.3 % (35 of 60)

1. Polycystin-2 Polypeptide

GSTAIGINDT YSEVKSDLAQ QKAEMELSDL IRKGYHKALV KLKLKKNTVD DISESLRQGG GKLNFDELRQ DLKGKGHTDA EIEAIFTKYD QDGDQELTEH EHQQMRDDLE KEREDLDLDH SSL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Polycystin-2 Polypeptide	[U-100% 13C; U-100% 15N]	0.5 mM
2	Ca2	natural abundance	5 mM
3	DSS	natural abundance	0.1 mM
4	potassium phosphate buffer	natural abundance	10 mM
5	NaCl	natural abundance	500 mM
6	DTE	natural abundance	2 mM
7	H20	natural abundance	90 %
8	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KLE, Strand ID: A Detail

Release date

2009-04-15

Citation

NMR-assignments of a cytosolic domain of the C-terminus of polycystin-2
Schumann, F.H., Hoffmeister, H., Schmidt, M., Bader, R., Besl, E., Witzgall, R., Kalbitzer, H.
Biomol. NMR Assign. (2009), 3, 141-144, PubMed 19636966 , DOI 10.1007/s12104-009-9160-x , Abstract

Related entities 1. Polycystin-2 Polypeptide, : 1 : 1 : 4 : 20 entities Detail

Interaction partners 1. Polycystin-2 Polypeptide, : 21 interactors Detail

More details for 21 interactors identified by 9 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC