BMRBj - BMREntryMaster

Found 1 Document (1.549 seconds)

BMRB: 16195

2LG1

Resonance assignments of the human AKAP13 PH domain and stabilizing DH helix

Authors

Sugawara, M., Whittaker, S.B.M., Bishop, S., Ball, L., Overduin, M.

Assembly

AKAP13-PH

Entity

1. AKAP13-PH (polymer, Thiol state: not present), 185 monomers, 21023.91 Da Detail

SMTKDNEVEQ EDLAQSLSLV KDVIGAVDSK VASYEKKVRL NEIYTKTDSK SIMRMKSGQM FAKEDLKRKK LVRDGSVFLK NAAGRLKEVQ AVLLTDILVF LQEKDQKYIF ASLDQKSTVI SLKKLIVREV AHEEKGLFLI SMGMTDPEMV EVHASSKEER NSWIQIIQDT INTLNRDEDE GIPSE

Formula weight

21023.91 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.6 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.6 % (2087 of 2206)	92.6 % (1070 of 1156)	96.9 % (825 of 851)	96.5 % (192 of 199)
Backbone	96.9 % (1072 of 1106)	96.8 % (363 of 375)	96.9 % (531 of 548)	97.3 % (178 of 183)
Sidechain	93.3 % (1192 of 1278)	90.5 % (707 of 781)	97.9 % (471 of 481)	87.5 % (14 of 16)
Aromatic	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (46 of 46)	100.0 % (1 of 1)
Methyl	96.4 % (216 of 224)	94.6 % (106 of 112)	98.2 % (110 of 112)

1. AKAP13-PH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 500uM AKAP13 extended PH domain in 50mM phosphate buffer at pH 7.0, 150mM NaCl, 0.02% NaN3, 10% D2O

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LG1, Strand ID: A Detail

Release date

2009-08-09

Citation

Resonance assignments of the human AKAP13-PH domain and stabilizing DH helix
Sugawara, M., Whittaker, S.B.M., Bishop, S., Ball, L., Overduin, M.
Biomol. NMR Assign. (2009), 3, 215-218, PubMed 19888694 , DOI 10.1007/s12104-009-9178-0 , Abstract

Related entities 1. AKAP13-PH, : 1 : 2 : 66 entities Detail

Interaction partners 1. AKAP13-PH, : 30 interactors Detail

More details for 30 interactors identified by 9 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 500uM AKAP13 extended PH domain in 50mM phosphate buffer at pH 7.0, 150mM NaCl, 0.02% NaN3, 10% D2O

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

2 3D HNCO

Instrument

Varian INOVA - 800 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 800 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

4 3D HN(CO)CA

Instrument

Varian INOVA - 800 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

5 3D HNCA

Instrument

Varian INOVA - 800 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

6 3D HNCACB

Instrument

Varian INOVA - 800 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

7 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

8 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

9 3D 1H-13C NOESY

Instrument

Varian INOVA - 900 MHz equipped with triple resonance cold probe with enhanced 13C and 1H sensitivity and z-axis gradiant.

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	Sodium azide	natural abundance	0.02 %
4	D2O	[U-99% 2H]	10 %
5	AKAP13-PH	[U-100% 13C; U-100% 15N]	0.5 mM

nullKeywords AKAP13, Brx, Lbc, NMR resonance assignment, PH domain

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Found 1 Document (1.549 seconds)

BMRB: 16195

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. AKAP13-PH, : 1 : 2 : 66 entities Detail

Interaction partners 1. AKAP13-PH, : 30 interactors Detail

Experiments performed 9 experiments Detail

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