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BMRB: 16214

2KGO

Zn finger protein YBIL from E.Coli; NESG target ET107; OCSP target EC0402

Authors

Gutmanas, A., Yee, A., Lemak, A., Fares, C., Guido, V., Arrowsmith, C.H.

Assembly

Zn finger protein YBIL

Entity

1. YBIL (polymer, Thiol state: all free), 108 monomers, 11919.00 Da Detail

MGSSHHHHHH SSGLVPRGSH MASGWANDDA VNEQINSTIE DAIARARGEI PRGESLDECE ECGAPIPQAR REAIPGVRLC IHCQQEKDLQ KPAYTGYNRR GSKDSQLR

2. ZN (non-polymer), 65.409 Da

Total weight

11984.409 Da

Max. entity weight

11919.0 Da

Source organism

Vitreoscilla beggiatoides

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.3 %, Completeness: 80.6 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.6 % (953 of 1183)	80.6 % (502 of 623)	79.6 % (356 of 447)	84.1 % (95 of 113)
Backbone	80.2 % (510 of 636)	80.5 % (177 of 220)	79.3 % (249 of 314)	82.4 % (84 of 102)
Sidechain	81.2 % (524 of 645)	80.6 % (325 of 403)	81.4 % (188 of 231)	100.0 % (11 of 11)
Aromatic	53.3 % (32 of 60)	53.3 % (16 of 30)	51.7 % (15 of 29)	100.0 % (1 of 1)
Methyl	92.9 % (78 of 84)	92.9 % (39 of 42)	92.9 % (39 of 42)

1. YBIL

MGSSHHHHHH SSGLVPRGSH MASGWANDDA VNEQINSTIE DAIARARGEI PRGESLDECE ECGAPIPQAR REAIPGVRLC IHCQQEKDLQ KPAYTGYNRR GSKDSQLR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
2	Zn sulfate	natural abundance	10 uM
3	DTT	[U-2H]	10 mM
4	TRIS	[U-2H]	10 mM
5	sodium chloride	natural abundance	300 mM
6	sodium azide	natural abundance	0.01 %
7	benzamidine	natural abundance	1 mM
8	Roche inhibitor	natural abundance	1 na
9	H20	natural abundance	90 %
10	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KGO, Strand ID: A Detail

Release date

2009-04-13

Citation

Zn-finger protein YBIL from E. Coli
Gutmanas, A., Yee, A., Lemak, A., Fares, C., Arrowsmith, C.H.
Not known

Related entities 1. YBIL, : 1 : 2 : 8 entities Detail

Interaction partners 1. YBIL, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC