Refined solution structure of des-pyro Glu brazzein
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.8 % (589 of 635) | 92.9 % (313 of 337) | 91.2 % (218 of 239) | 98.3 % (58 of 59) |
Backbone | 96.8 % (306 of 316) | 97.2 % (103 of 106) | 96.2 % (152 of 158) | 98.1 % (51 of 52) |
Sidechain | 89.2 % (331 of 371) | 90.9 % (210 of 231) | 86.5 % (115 of 133) | 85.7 % (6 of 7) |
Aromatic | 54.8 % (34 of 62) | 54.8 % (17 of 31) | 54.8 % (17 of 31) | |
Methyl | 100.0 % (28 of 28) | 100.0 % (14 of 14) | 100.0 % (14 of 14) |
1. wt-brazzein
DKCKKVYENY PVSKCQLANQ CNYDCKLDKH ARSGECFYDE KRNLQCICDY CEYSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 (±0.1) K, pH 5.2 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | wt-brazzein | [U-13C; U-15N] | 2 (±0.3) mM |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 (±0.1) K, pH 5.2 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | wt-brazzein | [U-15N] | 1 (±0.1) mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 (±0.1) K, pH 5.2 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | wt-brazzein | [U-15N] | 1 (±0.1) mM |