BMRBj - BMREntryMaster

Found 1 Document (1.185 seconds)

BMRB: 16249

2KHM

The carboxy-terminal non-repetitive domain of a spider dragline silk protein regulates nucleation of silk assembly

Authors

Hagn, F.X., Eisoldt, L., Hardy, J., Vendrely, C., Coles, M., Scheibel, T., Kessler, H.

Assembly

NR3 dimer

Entity

1. NR3 dimer (polymer, Thiol state: all disulfide bound), 140 monomers, 13326.71 × 2 Da Detail

MASMTGGQQM GRGSMGAASA AVSVGGYGPQ SSSAPVASAA ASRLSSPAAS SRVSSAVSSL VSSGPTNQAA LSNTISSVVS QVSASNPGLS GCDVLVQALL EVVSALVSIL GSSSIGQINY GASAQYTQMV GQSVAQALAG

Total weight

26653.42 Da

Max. entity weight

13326.71 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS92:SG	1:CYS92:SG

Source organism

Araneus diadematus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 96.3 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (1321 of 1372)	97.0 % (669 of 690)	95.1 % (506 of 532)	97.3 % (146 of 150)
Backbone	96.1 % (798 of 830)	97.9 % (285 of 291)	94.1 % (380 of 404)	98.5 % (133 of 135)
Sidechain	96.8 % (645 of 666)	96.2 % (384 of 399)	98.4 % (248 of 252)	86.7 % (13 of 15)
Aromatic	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	98.3 % (171 of 174)	96.6 % (84 of 87)	100.0 % (87 of 87)

1. NR3

MASMTGGQQM GRGSMGAASA AVSVGGYGPQ SSSAPVASAA ASRLSSPAAS SRVSSAVSSL VSSGPTNQAA LSNTISSVVS QVSASNPGLS GCDVLVQALL EVVSALVSIL GSSSIGQINY GASAQYTQMV GQSVAQALAG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	NR3	natural abundance	0.6 ~ 1.0 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	NR3	[U-99% 13C; U-99% 15N]	1 mM
10	NR3	natural abundance	1 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
13	NR3	[U-99% 15N]	0.2 ~ 1.0 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 2KHM, Strand ID: A, B Detail

Release date

2010-05-06

Citation

A conserved spider silk domain acts as a molecular switch that controls fibre assembly
Hagn, F., Eisoldt, L., Hardy, J.G., Vendrely, C., Coles, M., Scheibel, T., Kessler, H.
Nature (2010), 465, 239-242, PubMed 20463741 , DOI 10.1038/nature08936 , Abstract

Related entities 1. NR3 dimer, : 1 : 1 entities Detail

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
13	NR3	[U-99% 15N]	0.2 ~ 1.0 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

3 3D HNCO

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

4 3D HNCA

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

5 3D HNCACB

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

6 3D CBCA(CO)NH

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

7 3D HN(CO)CA

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

8 3D HN(CA)CO

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

9 3D HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

10 3D HNHA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
13	NR3	[U-99% 15N]	0.2 ~ 1.0 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

11 3D HNHB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
13	NR3	[U-99% 15N]	0.2 ~ 1.0 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

12 3D CCH-COSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

13 3D C(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

14 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
13	NR3	[U-99% 15N]	0.2 ~ 1.0 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

15 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

16 3D CNH NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

17 3D NNH NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
13	NR3	[U-99% 15N]	0.2 ~ 1.0 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

18 3D CCH NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 1mM NR3 in 10mM sodium phosphate pH6.0 1% trifluoroethanol

#	Name	Isotope labeling	Concentration
1	NR3	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	trifluoroethanol	natural abundance	1 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

19 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	NR3	natural abundance	0.6 ~ 1.0 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

20 filtered NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	NR3	[U-99% 13C; U-99% 15N]	1 mM
10	NR3	natural abundance	1 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_16249_2khm.nef
Input source #2: Coordindates	2khm.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
A:92:CYS:SG	B:92:CYS:SG	oxidized, CA 61.44, CB 44.53 ppm	unknown	2.02

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MASMTGGQQMGRGSMGAASAAVSVGGYGPQSSSAPVASAAASRLSSPAASSRVSSAVSSLVSSGPTNQAALSNTISSVVSQVSASNPGLSGCDVLVQALL
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MASMTGGQQMGRGSMGAASAAVSVGGYGPQSSSAPVASAAASRLSSPAASSRVSSAVSSLVSSGPTNQAALSNTISSVVSQVSASNPGLSGCDVLVQALL

-------110-------120-------130-------140
EVVSALVSILGSSSIGQINYGASAQYTQMVGQSVAQALAG
||||||||||||||||||||||||||||||||||||||||
EVVSALVSILGSSSIGQINYGASAQYTQMVGQSVAQALAG

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MASMTGGQQMGRGSMGAASAAVSVGGYGPQSSSAPVASAAASRLSSPAASSRVSSAVSSLVSSGPTNQAALSNTISSVVSQVSASNPGLSGCDVLVQALL
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MASMTGGQQMGRGSMGAASAAVSVGGYGPQSSSAPVASAAASRLSSPAASSRVSSAVSSLVSSGPTNQAALSNTISSVVSQVSASNPGLSGCDVLVQALL

-------110-------120-------130-------140
EVVSALVSILGSSSIGQINYGASAQYTQMVGQSVAQALAG
||||||||||||||||||||||||||||||||||||||||
EVVSALVSILGSSSIGQINYGASAQYTQMVGQSVAQALAG

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	140	0	0	100.0
B	B	140	0	0	100.0

Content subtype: combined_16249_2khm.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	1		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1349		99.3 (chain: A, length: 140)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	1377 (1)	noe	87.1 (chain: A, length: 140), 32.9 (chain: B, length: 140)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 140, Sequence coverage: 99.3%

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MASMTGGQQMGRGSMGAASAAVSVGGYGPQSSSAPVASAAASRLSSPAASSRVSSAVSSLVSSGPTNQAALSNTISSVVSQVSASNPGLSGCDVLVQALL
 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
.ASMTGGQQMGRGSMGAASAAVSVGGYGPQSSSAPVASAAASRLSSPAASSRVSSAVSSLVSSGPTNQAALSNTISSVVSQVSASNPGLSGCDVLVQALL

-------110-------120-------130-------140
EVVSALVSILGSSSIGQINYGASAQYTQMVGQSVAQALAG
||||||||||||||||||||||||||||||||||||||||
EVVSALVSILGSSSIGQINYGASAQYTQMVGQSVAQALAG

Total number of assignments
- ¹H chemical shifts: 684
- ¹³C chemical shifts: 517
- ¹⁵N chemical shifts: 148

Completeness of assignments

Entity_assemble_ID: A

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
27	TYR	HH	10.87
30	GLN	CD	180.8
52	ARG	HH11	6.39
52	ARG	HH12	6.39
52	ARG	NH1	84.85
67	ASN	CG	176.9
68	GLN	CD	179.6
72	SER	HG	5.21
73	ASN	CG	176.3
74	THR	HG1	5.04
81	GLN	CD	180.3
85	SER	HG	4.71
86	ASN	CG	178.8
90	SER	HG	4.7
97	GLN	CD	179.88
108	SER	HG	4.43
112	SER	HG	4.19
113	SER	HG	4.2
120	TYR	HH	10.92
123	SER	HG	5.43
125	GLN	CD	180.0
126	TYR	HH	10.62
127	THR	HG1	4.69
128	GLN	CD	180.3
132	GLN	CD	180.1
136	GLN	CD	180.2

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	690	670	97.1
¹³C chemical shifts	532	506	95.1
¹⁵N chemical shifts	153	147	96.1

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	291	287	98.6
¹³C chemical shifts	280	258	92.1
¹⁵N chemical shifts	135	133	98.5

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	399	383	96.0
¹³C chemical shifts	252	248	98.4
¹⁵N chemical shifts	18	14	77.8

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	92	88	95.7
¹³C chemical shifts	92	91	98.9

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	12	12	100.0
¹³C chemical shifts	12	12	100.0

Redox state of CYS
Peptide bond of PRO
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: A

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 140, Sequence coverage: 87.1%
  - Entity_assembly_ID: B, Chain length: 140, Sequence coverage: 32.9%
- Total number of restraints: 1376
- Weight of restraints: 1.0 (1376)
- Potential type of restraints: square-well-parabolic (1376)
- Range of distance target values: 0.0, 7.5 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map

Keywords biopolymers, fibers, NMR spectroscopy, protein folding, structure, alpha helix, homodimer, swapped

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Found 1 Document (1.185 seconds)

BMRB: 16249

Sample #1

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. NR3 dimer, : 1 : 1 entities Detail

Experiments performed 20 experiments Detail

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NMR combined restraints 3 contents Detail