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BMRB: 16250

2KHN

NMR solution structure of the EH 1 domain from human intersectin-1 protein. Northeast Structural Genomics Consortium target HR3646e.

Authors

Mills, J.L., Ghosh, A., Garcia, E., Zhang, Q., Shastry, R., Foote, E.L., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

human intersectin-1

Entity

1. human intersectin-1 (polymer, Thiol state: not present), 121 monomers, 13596.40 Da Detail

MGHHHHHHSH MAQFPTPFGG SLDTWAITVE ERAKHDQQFH SLKPISGFIT GDQARNFFFQ SGLPQPVLAQ IWALADMNND GRMDQVEFSI AMKLIKLKLQ GYQLPSALPP VMKQQPVAIS S

Formula weight

13596.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.3 %, Completeness: 76.6 %, Completeness (bb): 75.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.6 % (1105 of 1442)	83.1 % (625 of 752)	65.4 % (367 of 561)	87.6 % (113 of 129)
Backbone	75.4 % (534 of 708)	88.4 % (213 of 241)	62.3 % (221 of 355)	89.3 % (100 of 112)
Sidechain	79.2 % (671 of 847)	80.6 % (412 of 511)	77.1 % (246 of 319)	76.5 % (13 of 17)
Aromatic	43.9 % (65 of 148)	47.3 % (35 of 74)	38.9 % (28 of 72)	100.0 % (2 of 2)
Methyl	93.1 % (108 of 116)	93.1 % (54 of 58)	93.1 % (54 of 58)

1. HR3646E

MGHHHHHHSH MAQFPTPFGG SLDTWAITVE ERAKHDQQFH SLKPISGFIT GDQARNFFFQ SGLPQPVLAQ IWALADMNND GRMDQVEFSI AMKLIKLKLQ GYQLPSALPP VMKQQPVAIS S

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	HR3646E	[U-98% 13C; U-98% 15N]	0.87 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H20	natural abundance	90 %
8	D20	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	HR3646E	[U-5% 13C; U-98% 15N]	0.81 mM
10	MES	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	10 mM
14	sodium azide	natural abundance	0.02 %
15	H20	natural abundance	90 %
16	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KHN, Strand ID: A Detail

Release date

2009-06-10

Citation

NMR solution structure of the EH 1 domain from human intersectin-1 protein. Northeast Structural Genomics Consortium target HR3646e
Mills, J.L., Ghosh, A., Garcia, E., Zhang, Q., Shastry, R., Foote, E.L., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.
Not known

Related entities 1. human intersectin-1, : 1 : 4 : 27 entities Detail

Interaction partners 1. human intersectin-1, : 41 interactors Detail

More details for 41 interactors identified by 16 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC