BMRB: 16252

1H, 13C, 15N Chemical shift assignments of A13 homeodomain

Authors

Ames, J.

Assembly

A13

Entity

1. A13 (polymer, Thiol state: not present), 73 monomers, 8866.286 Da Detail

MSHMLEGRKK RVPYTKVQLK ELEREYATNK FITKDKRRRI SATTNLSERQ VTIWFQNRRV KEKKVINKLK TTS

Formula weight

8866.286 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 75.2 %, Completeness (bb): 95.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.2 % (708 of 942)	80.4 % (406 of 505)	65.0 % (232 of 357)	87.5 % (70 of 80)
Backbone	95.2 % (415 of 436)	94.5 % (138 of 146)	95.4 % (208 of 218)	95.8 % (69 of 72)
Sidechain	62.8 % (363 of 578)	74.9 % (269 of 359)	44.1 % (93 of 211)	12.5 % (1 of 8)
Aromatic	53.8 % (28 of 52)	100.0 % (26 of 26)	4.0 % (1 of 25)	100.0 % (1 of 1)
Methyl	69.7 % (53 of 76)	94.7 % (36 of 38)	44.7 % (17 of 38)

1. A13

MSHMLEGRKK RVPYTKVQLK ELEREYATNK FITKDKRRRI SATTNLSERQ VTIWFQNRRV KEKKVINKLK TTS

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 285 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	A13	[U-98% 13C; U-98% 15N]		0.5 mM
2	H2O	natural abundance		95 %
3	D2O	natural abundance		5 %

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LACS Plot; CA

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.44 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2L7Z, Strand ID: A Detail

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Release date

2009-07-09

Citation

(1)H, (15)N, and (13)C chemical shift assignments of mouse HOXA13 DNA binding domain
Zhang, Y., Thornburg, C.K., Stadler, H., Ames, J.B.
Biomol. NMR Assign. (2009), 3, 199-201, PubMed 19888690 , DOI 10.1007/s12104-009-9174-4 , Abstract

Related entities 1. A13, : 1 : 8 : 169 entities Detail

Interaction partners 1. A13, : 1 interactors Detail

Experiments performed 5 experiments Detail