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BMRB: 16255

2KHV

Solution Structure Of Protein Nmul_A0922 From Nitrosospira multiformis. Northeast Structural Genomics Consortium Target Hr5524a.

Authors

Wu, Y., Mills, J.L., Wang, D., Ciccosanti, C., Sukumaran, D.K., Jiang, M., Garcia, E., Nair, R., Rost, B., Swapna, G., Acton, T., Xiao, R., Everett, J., Montelione, G., Szyperski, T.

Assembly

Nmul A0922

Entity

1. Nmul A0922 (polymer, Thiol state: all free), 106 monomers, 12375.87 Da Detail

MTFSECAALY IKAHRSSWKN TKHADQWTNT IKTYCGPVIG PLSVQDVDTK LIMKVLDPIW EQKPETASRL RGRIESVLDW ATVRGYREGD NPARWRGYLE HHHHHH

Formula weight

12375.87 Da

Source organism

Nitrosospira multiformis ATCC 25196

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 85.7 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.7 % (1081 of 1261)	92.8 % (609 of 656)	74.2 % (366 of 493)	94.6 % (106 of 112)
Backbone	80.2 % (502 of 626)	93.4 % (199 of 213)	66.7 % (208 of 312)	94.1 % (95 of 101)
Sidechain	91.4 % (672 of 735)	92.6 % (410 of 443)	89.3 % (251 of 281)	100.0 % (11 of 11)
Aromatic	74.6 % (100 of 134)	82.1 % (55 of 67)	64.5 % (40 of 62)	100.0 % (5 of 5)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. Nmul A0922

MTFSECAALY IKAHRSSWKN TKHADQWTNT IKTYCGPVIG PLSVQDVDTK LIMKVLDPIW EQKPETASRL RGRIESVLDW ATVRGYREGD NPARWRGYLE HHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.9 mM
2	H20	natural abundance	90.0 %
3	D20	natural abundance	10.0 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-5% 13C; U-100% 15N]	1.0 mM
5	H20	natural abundance	90.0 %
6	D20	natural abundance	10.0 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KHV, Strand ID: A Detail

Release date

2009-05-19

Citation

Solution Structure Of Protein Nmul_A0922 From Nitrosospira multiformis. Northeast Structural Genomics Consortium Target Hr5524a
Wu, Y., Mills, J., Wang, D., Ciccosanti, C., Sukumaran, D., Jiang, M., Garcia, E., Nair, R., Rost, B., Swapna, G., Acton, T., Everett, J., Montelione, G., Szyperski, T.
Not known

Related entities 1. Nmul A0922, : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC