BMRB: 16269

n-NafY. N-terminal domain of NafY

Authors

Phillips, A.H., Hernandez, J.A., Erbil, K., Pelton, J.G., Wemmer, D.E., Rubio, L.M.

Assembly

n-NafY

Entity

1. n-NafY (polymer, Thiol state: not present), 98 monomers, 10470.89 Da Detail

GHMVTPVNMS RETALRIALA ARALPGTTVG QLLEILHQRI EGPLTEESLQ GVSVTDLKIG LAGSEEDVDM LDTPMSALKD AVRILWGEAE VDSLPQPV

Formula weight

10470.89 Da

Source organism

Azotobacter vinelandii

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.7 %, Completeness: 67.8 %, Completeness (bb): 71.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	67.8 % (731 of 1078)	73.3 % (406 of 554)	57.3 % (244 of 426)	82.7 % (81 of 98)
Backbone	71.7 % (413 of 576)	85.4 % (169 of 198)	57.3 % (164 of 286)	87.0 % (80 of 92)
Sidechain	65.7 % (389 of 592)	66.6 % (237 of 356)	65.7 % (151 of 230)	16.7 % (1 of 6)
Aromatic	35.0 % (7 of 20)	60.0 % (6 of 10)	0.0 % (0 of 9)	100.0 % (1 of 1)
Methyl	70.1 % (101 of 144)	72.2 % (52 of 72)	68.1 % (49 of 72)

1. n-NafY

GHMVTPVNMS RETALRIALA ARALPGTTVG QLLEILHQRI EGPLTEESLQ GVSVTDLKIG LAGSEEDVDM LDTPMSALKD AVRILWGEAE VDSLPQPV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	n-NafY	[U-100% 13C; U-100% 15N]		1 mM
2	NaCl	natural abundance		140 mM
3	KCl	natural abundance		2.7 mM
4	Na2HPO4	natural abundance		10 mM
5	KH2PO4	natural abundance		1.8 mM
6	EDTA	natural abundance		2 mM
7	NaN3	natural abundance		0.02 % w/v

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
8	n-NafY	[U-100% 13C; U-100% 15N]		1 mM
9	NaCl	natural abundance		140 mM
10	KCl	natural abundance		2.7 mM
11	Na2HPO4	natural abundance		10 mM
12	KH2PO4	natural abundance		1.8 mM
13	EDTA	natural abundance		2 mM
14	NaN3	natural abundance		0.02 % w/v

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LACS Plot; CA

Referencing offset: 0.51 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.51 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KIC, Strand ID: A Detail

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Release date

2012-08-05

Citation

Biological activity and solution structure of the apo-dinitrogenase binding domain of NafY
Phillips, A.H., Hernandez, J.A., Erbil, K., Pelton, J.G., Wemmer, D.E., Rubio, L.M.
Not known

Related entities 1. n-NafY, : 1 : 5 entities Detail

Experiments performed 1 experiments Detail