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BMRB: 16271

2KIE

A PH domain within OCRL bridges clathrin mediated membrane trafficking to phosphoinositide metabolis

Authors

Mao, Y., Balkin, D.M., Zoncu, R., Erdmann, K., Tomasini, L., Hu, F., Jin, M.M., Hodsdon, M.E., De Camilli, P.

Assembly

OCRL bridges clathrin-mediated membrane

Entity

1. OCRL bridges clathrin-mediated membrane (polymer, Thiol state: free and disulfide bound), 372 monomers, 41525.20 Da Detail

GPLGSMEPPL PVGAQPLATV EGMEMKGPLR EPCALTLAQR NGQYELIIQL HEKEQHVQDI IPINSHFRCV QEAEETLLID IASNSGCKIR VQGDWIRERR FEIPDEEHCL KFLSAVLAAQ KAQSGPLGSM EPPLPVGAQP LATVEGMEMK GPLREPCALT LAQRNGQYEL IIQLHEKEQH VQDIIPINSH FRCVQEAEET LLIDIASNSG CKIRVQGDWI RERRFEIPDE EHCLKFLSAV LAAQKAQSGP LGSMEPPLPV GAQPLATVEG MEMKGPLREP CALTLAQRNG QYELIIQLHE KEQHVQDIIP INSHFRCVQE AEETLLIDIA SNSGCKIRVQ GDWIRERRFE IPDEEHCLKF LSAVLAAQKA QS

Formula weight

41525.2 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS87:SG	1:CYS109:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 46.8 %, Completeness: 39.1 %, Completeness (bb): 38.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	39.1 % (1698 of 4341)	41.9 % (956 of 2283)	34.5 % (576 of 1671)	42.9 % (166 of 387)
Backbone	38.5 % (838 of 2178)	43.9 % (325 of 741)	33.2 % (362 of 1092)	43.8 % (151 of 345)
Sidechain	39.9 % (1003 of 2511)	40.9 % (631 of 1542)	38.5 % (357 of 927)	35.7 % (15 of 42)
Aromatic	34.8 % (69 of 198)	41.4 % (41 of 99)	28.1 % (27 of 96)	33.3 % (1 of 3)
Methyl	41.5 % (177 of 426)	42.7 % (91 of 213)	40.4 % (86 of 213)

1. PH domain

Show sample condition

Sample

Solvent system potasium phosphate, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	entity			1 mM
2	potassium phosphate	natural abundance		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl proton	0.0 ppm	internal	direct	1.0

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl proton	0.0 ppm	internal	direct	1.0

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl proton	0.0 ppm	internal	direct	1.0

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KIE, Strand ID: A Detail

Release date

2009-06-24

Citation

A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism
Mao, Y., Balkin, D.M., Zoncu, R., Erdmann, K.S., Tomasini, L., Hu, F., Jin, M.M., Hodsdon, M.E., De Camilli, P.
EMBO J. (2009), 28, 1831-1842, PubMed 19536138 , DOI 10.1038/emboj.2009.155 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

  BMRB: 16273 released on 2009-06-24
    Title A PH domain within OCRL bridges clathrin mediated membrane trafficking to phosphoinositide metabolism
  Swiss-Prot: Q8K337 released on 2012-01-25
    Title I5P2_MOUSE Entity Type II inositol 1,4,5-trisphosphate 5-phosphatase
  Swiss-Prot: Q01968 released on 1993-07-01
    Title OCRL_HUMAN Entity Inositol polyphosphate 5-phosphatase OCRL-1

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC