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BMRB: 16273

2KIG

A PH domain within OCRL bridges clathrin mediated membrane trafficking to phosphoinositide metabolism

Authors

Mao, Y., Hodsdon, M.E., De Camilli, P.

Assembly

OCRL bridges clathrin-mediated membrane

Entity

1. OCRL bridges clathrin-mediated membrane (polymer, Thiol state: all free), 483 monomers, 53928.09 Da Detail

GPLGSMDQSV AIQETLVEGE YCVIAVQGVL CKGDSRQSRL LGLVRYRLEN DAQEHALFLY THRRMAITGD DVSLDQIVPL SKDFMLEEVS PDGELYILGS DVTVQLNTAE LKLVFQLPFG SHTRTFLQEV ARACPGFDPE TRDPEFEWLS RHTCAEPDAE SGPLGSMDQS VAIQETLVEG EYCVIAVQGV LCKGDSRQSR LLGLVRYRLE NDAQEHALFL YTHRRMAITG DDVSLDQIVP LSKDFMLEEV SPDGELYILG SDVTVQLNTA ELKLVFQLPF GSHTRTFLQE VARACPGFDP ETRDPEFEWL SRHTCAEPDA ESGPLGSMDQ SVAIQETLVE GEYCVIAVQG VLCKGDSRQS RLLGLVRYRL ENDAQEHALF LYTHRRMAIT GDDVSLDQIV PLSKDFMLEE VSPDGELYIL GSDVTVQLNT AELKLVFQLP FGSHTRTFLQ EVARACPGFD PETRDPEFEW LSRHTCAEPD AES

Formula weight

53928.09 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 54.5 %, Completeness: 44.1 %, Completeness (bb): 48.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.1 % (2425 of 5499)	43.4 % (1232 of 2841)	43.7 % (946 of 2163)	49.9 % (247 of 495)
Backbone	48.3 % (1377 of 2850)	47.7 % (465 of 975)	48.0 % (679 of 1416)	50.8 % (233 of 459)
Sidechain	40.9 % (1268 of 3099)	41.1 % (767 of 1866)	40.7 % (487 of 1197)	38.9 % (14 of 36)
Aromatic	36.2 % (141 of 390)	40.0 % (78 of 195)	32.3 % (62 of 192)	33.3 % (1 of 3)
Methyl	44.7 % (255 of 570)	44.9 % (128 of 285)	44.6 % (127 of 285)

1. PH domain

Show sample condition

Sample

Solvent system potasium phosphate, Pressure 1.0 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	entity			0.0 ~ 0.0 mM
2	potassium phosphate	natural abundance		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl carbon	0.0 ppm	internal	direct	1.0

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl carbon	0.0 ppm	internal	direct	1.0

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl carbon	0.0 ppm	internal	direct	1.0

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl carbon	0.0 ppm	internal	direct	1.0

Referencing offset: 0.38 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KIG, Strand ID: A Detail

Release date

2009-06-24

Citation

A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism
Mao, Y., Balkin, D.M., Zoncu, R., Erdmann, K.S., Tomasini, L., Hu, F., Jin, M.M., Hodsdon, M.E., De Camilli, P.
EMBO J. (2009), 28, 1831-1842, PubMed 19536138 , DOI 10.1038/emboj.2009.155 , Abstract

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC