BMRB: 16293

Solution structure of CA150 FF1 domain and FF1-FF2 interdomain linker

Authors

Murphy, J.M., Hansen, D., Wiesner, S., Muhandiram, D., Borg, M., Smith, M.J., Sicheri, F., Kay, L.E., Forman-Kay, J.D., Pawson, T.

Assembly

CA150 FF1+linker

Entity

1. CA150 FF1+linker (polymer, Thiol state: not present), 71 monomers, 8559.810 Da Detail

GAMGSLEARM KQFKDMLLER GVSAFSTWEK ELHKIVFDPR YLLLNPKERK QVFDQYVKTR AEEERREKKN K

Formula weight

8559.81 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.6 %, Completeness: 77.6 %, Completeness (bb): 71.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.6 % (702 of 905)	86.8 % (421 of 485)	76.8 % (265 of 345)	21.3 % (16 of 75)
Backbone	71.3 % (301 of 422)	95.8 % (137 of 143)	70.5 % (148 of 210)	23.2 % (16 of 69)
Sidechain	85.1 % (469 of 551)	83.0 % (284 of 342)	91.1 % (185 of 203)	0.0 % (0 of 6)
Aromatic	75.0 % (54 of 72)	86.1 % (31 of 36)	65.7 % (23 of 35)	0.0 % (0 of 1)
Methyl	98.3 % (59 of 60)	96.7 % (29 of 30)	100.0 % (30 of 30)

1. CA150 FF1+linker

GAMGSLEARM KQFKDMLLER GVSAFSTWEK ELHKIVFDPR YLLLNPKERK QVFDQYVKTR AEEERREKKN K

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 285 K, pH 6.5, Details 3D data collected for protein at 1-1.7mM in PBS pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	CA150_FF1+linker	[U-99% 13C; U-99% 15N]		1.0 ~ 1.7 mM
2	H20	natural abundance		95 %
3	D20	natural abundance		5 %

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Chem. Shift Complete2

Sequence coverage: 98.6 %, Completeness: 68.1 %, Completeness (bb): 63.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	68.1 % (1232 of 1810)	85.8 % (832 of 970)	45.2 % (312 of 690)	58.7 % (88 of 150)
Backbone	63.9 % (539 of 844)	94.8 % (271 of 286)	44.3 % (186 of 420)	59.4 % (82 of 138)
Sidechain	69.3 % (764 of 1102)	80.8 % (553 of 684)	50.5 % (205 of 406)	50.0 % (6 of 12)
Aromatic	56.3 % (81 of 144)	79.2 % (57 of 72)	32.9 % (23 of 70)	50.0 % (1 of 2)
Methyl	74.2 % (89 of 120)	98.3 % (59 of 60)	50.0 % (30 of 60)

1. CA150 FF1+linker

GAMGSLEARM KQFKDMLLER GVSAFSTWEK ELHKIVFDPR YLLLNPKERK QVFDQYVKTR AEEERREKKN K

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 285 K, pH 6.5, Details 3D data collected for protein at 1-1.7mM in PBS pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	CA150_FF1+linker	[U-99% 13C; U-99% 15N]		1.0 ~ 1.7 mM
2	H20	natural abundance		95 %
3	D20	natural abundance		5 %

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Protein Blocks Logo

Calculated from 7 models in PDB: 2KIS, Strand ID: A Detail

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Release date

2009-09-03

Citation

Structural studies of FF domains of the transcription factor CA150 provide insights into the organization of FF domain tandem arrays
Murphy, J.M., Hansen, D., Wiesner, S., Muhandiram, D., Borg, M., Smith, M.J., Sicheri, F., Kay, L.E., Forman-Kay, J.D., Pawson, T.
J. Mol. Biol. (2009), 393, 409-424, PubMed 19715701 , DOI 10.1016/j.jmb.2009.08.049 , Abstract

Related entities 1. CA150 FF1+linker, : 1 : 55 entities Detail

Interaction partners 1. CA150 FF1+linker, : 7 interactors Detail

Experiments performed 5 experiments Detail