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BMRB: 16295

2KIT

The solution struture of the reduced yeast TOR1 FATC domain bound to DPC micelles at 298K

Authors

Assembly

y1fatc

Entity

1. y1fatc (polymer, Thiol state: all free), 33 monomers, 3960.446 Da Detail

NELDVPEQVD KLIQQATSIE RLCQHYIGWC PFW

Formula weight

3960.446 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.1 %, Completeness (bb): 81.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.1 % (353 of 410)	92.1 % (197 of 214)	75.9 % (120 of 158)	94.7 % (36 of 38)
Backbone	81.4 % (158 of 194)	96.9 % (63 of 65)	67.3 % (66 of 98)	93.5 % (29 of 31)
Sidechain	91.5 % (227 of 248)	89.9 % (134 of 149)	93.5 % (86 of 92)	100.0 % (7 of 7)
Aromatic	73.9 % (34 of 46)	73.9 % (17 of 23)	71.4 % (15 of 21)	100.0 % (2 of 2)
Methyl	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)

1. y1fatc

NELDVPEQVD KLIQQATSIE RLCQHYIGWC PFW

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2KIT, Strand ID: A Detail

Release date

2011-10-06

Citation

Structural basis for the association of the redox-sensitive target of rapamycin FATC domain with membrane-mimetic micelles
Dames, S.A.
J. Biol. Chem. (2010), 285, 7766-7775, PubMed 20042596 , DOI 10.1074/jbc.M109.058404 , Abstract

Related entities 1. y1fatc, : 1 : 4 : 97 entities Detail

Interaction partners 1. y1fatc, : 71 interactors Detail

More details for 71 interactors identified by 11 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

2 2D 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

3 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

4 2D 1H-13C HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

5 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

6 3D HCCH-TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

7 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

8 3D 1H-13C NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

9 3D HACAHB-COSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

10 3D HNHB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

11 {15N} SED 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

12 {13C'} SED 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

13 15N T1

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

14 15N T2

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

15 {1H}-15N-NOE

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	y1fatc	[U-13C; U-15N]		0.5 mM
2	Tris	natural abundance		50 mM
3	NaCl	natural abundance		100 mM
4	TCEP	natural abundance		20 mM
5	dodecylphophocholine (DPC)	natural abundance		170 mM

nullKeywords dodecylphospholine, FATC, lipid-binding motif, micelle, redox-state, target of rapamycin, TOR1, dpc micelle, membrane-mimetic, protein