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BMRB: 16297

2KIV

AIDA-1 SAM domain tandem

Authors

Donaldson, L.W., Kurabi, A.

Assembly

AIDA-1 SAM domain tandem

Entity

1. AIDA-1 SAM domain tandem (polymer, Thiol state: all free), 148 monomers, 16647.95 Da Detail

MHHHHHHLVP RGSVQTVGQW LESIGLPQYE NHLMANGFDN VQAMGSNVME DQDLLEIGIL NSGHRQRILQ AIQLLPKMRP IGHDGAHPTS VAEWLDSIEL GDYTKAFLIN GYTSMDLLKK IAEVELINVL KINLIGHRKR ILASLGDR

Formula weight

16647.95 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.5 %, Completeness: 74.6 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.6 % (1296 of 1737)	72.6 % (655 of 902)	76.7 % (517 of 674)	77.0 % (124 of 161)
Backbone	82.2 % (722 of 878)	81.6 % (248 of 304)	82.8 % (357 of 431)	81.8 % (117 of 143)
Sidechain	69.1 % (687 of 994)	68.1 % (407 of 598)	72.2 % (273 of 378)	38.9 % (7 of 18)
Aromatic	54.5 % (61 of 112)	55.4 % (31 of 56)	51.9 % (28 of 54)	100.0 % (2 of 2)
Methyl	88.3 % (166 of 188)	89.4 % (84 of 94)	87.2 % (82 of 94)

1. AIDA-1 SAM domain

MHHHHHHLVP RGSVQTVGQW LESIGLPQYE NHLMANGFDN VQAMGSNVME DQDLLEIGIL NSGHRQRILQ AIQLLPKMRP IGHDGAHPTS VAEWLDSIEL GDYTKAFLIN GYTSMDLLKK IAEVELINVL KINLIGHRKR ILASLGDR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.1 mM
2	D2O	natural abundance	10 %
3	H2O	natural abundance	90 %
4	sodium azide	natural abundance	0.05 %
5	sodium phosphate	natural abundance	5 mM
6	sodium chloride	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KIV, Strand ID: A Detail

Release date

2009-09-03

Citation

A nuclear localization signal at the SAM-SAM domain interface of AIDA-1 suggests a requirement for domain uncoupling prior to nuclear import
Kurabi, A., Brener, S., Mobli, M., Kwan, J.J., Donaldson, L.W.
J. Mol. Biol. (2009), 392, 1168-1177, PubMed 19666031 , DOI 10.1016/j.jmb.2009.08.004 , Abstract

Related entities 1. AIDA-1 SAM domain tandem, : 1 : 2 : 65 entities Detail

Interaction partners 1. AIDA-1 SAM domain tandem, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC