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Backbone 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH8
Authors
Serniwka, S.A., Shaw, G.S.
Assembly
UbcH8
Entity
1. UbcH8 (polymer, Thiol state: all free), 152 monomers, 17605.16 Da Detail

MASMRVVKEL EDLQKKPPPY LRNLSSDDAN VLVWHALLLP DQPPYHLKAF NLRISFPPEY PFKPPMIKFT TKIYHPNVDE NGQICLPIIS SENWKPSTKT SQVLEALNVL VNRPNIREPL RMDLADLLTQ NPELFRKNAE EFTLRFGVDR PS


Formula weight
17605.16 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.7 %, Completeness: 57.9 %, Completeness (bb): 86.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All57.9 % (1086 of 1876)46.8 % (464 of 991)65.5 % (480 of 733)93.4 % (142 of 152)
Backbone86.0 % (753 of 876)72.2 % (208 of 288)91.2 % (414 of 454)97.8 % (131 of 134)
Sidechain41.2 % (474 of 1150)36.4 % (256 of 703)48.3 % (207 of 429)61.1 % (11 of 18)
Aromatic 0.0 % (0 of 138) 0.0 % (0 of 69) 0.0 % (0 of 67) 0.0 % (0 of 2)
Methyl59.4 % (101 of 170)63.5 % (54 of 85)55.3 % (47 of 85)

1. UbcH8

MASMRVVKEL EDLQKKPPPY LRNLSSDDAN VLVWHALLLP DQPPYHLKAF NLRISFPPEY PFKPPMIKFT TKIYHPNVDE NGQICLPIIS SENWKPSTKT SQVLEALNVL VNRPNIREPL RMDLADLLTQ NPELFRKNAE EFTLRFGVDR PS

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4


#NameIsotope labelingTypeConcentration
1UbcH8[U-100% 13C; U-100% 15N]0.5 mM
2sodium phosphatenatural abundance20 mM
3EDTAnatural abundance1 mM
4DTTnatural abundance1 mM
5sodium chloridenatural abundance150 mM
6Argininenatural abundance50 mM
7Glutamic Acidnatural abundance50 mM
8H2Onatural abundance90 %
9D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.3 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.3 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.15 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 16 models in PDB: 2KJH, Strand ID: A Detail


Release date
2009-10-13
Citation 1
The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site
Serniwka, S.A., Shaw, G.S.
Biochemistry (2009), 48, 12169-12179, PubMed 19928833 , DOI 10.1021/bi901686j ,
Citation 2
1H, 13C and 15N resonance assignments for the human E2 conjugating enzyme, UbcH7
Serniwka, S.A., Shaw, G.S.
Biomol. NMR Assign. (2008), 2, 21-23, PubMed 19636915 , DOI 10.1007/s12104-007-9074-4 ,
Related entities 1. UbcH8, : 1 : 1 : 8 : 287 entities Detail
Interaction partners 1. UbcH8, : 30 interactors Detail
Experiments performed 6 experiments Detail
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