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BMRB: 16321

2KJH

Backbone 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH8

Authors

Serniwka, S.A., Shaw, G.S.

Assembly

UbcH8

Entity

1. UbcH8 (polymer, Thiol state: all free), 152 monomers, 17605.16 Da Detail

MASMRVVKEL EDLQKKPPPY LRNLSSDDAN VLVWHALLLP DQPPYHLKAF NLRISFPPEY PFKPPMIKFT TKIYHPNVDE NGQICLPIIS SENWKPSTKT SQVLEALNVL VNRPNIREPL RMDLADLLTQ NPELFRKNAE EFTLRFGVDR PS

Formula weight

17605.16 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 57.9 %, Completeness (bb): 86.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	57.9 % (1086 of 1876)	46.8 % (464 of 991)	65.5 % (480 of 733)	93.4 % (142 of 152)
Backbone	86.0 % (753 of 876)	72.2 % (208 of 288)	91.2 % (414 of 454)	97.8 % (131 of 134)
Sidechain	41.2 % (474 of 1150)	36.4 % (256 of 703)	48.3 % (207 of 429)	61.1 % (11 of 18)
Aromatic	0.0 % (0 of 138)	0.0 % (0 of 69)	0.0 % (0 of 67)	0.0 % (0 of 2)
Methyl	59.4 % (101 of 170)	63.5 % (54 of 85)	55.3 % (47 of 85)

1. UbcH8

MASMRVVKEL EDLQKKPPPY LRNLSSDDAN VLVWHALLLP DQPPYHLKAF NLRISFPPEY PFKPPMIKFT TKIYHPNVDE NGQICLPIIS SENWKPSTKT SQVLEALNVL VNRPNIREPL RMDLADLLTQ NPELFRKNAE EFTLRFGVDR PS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	UbcH8	[U-100% 13C; U-100% 15N]	0.5 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	DTT	natural abundance	1 mM
5	sodium chloride	natural abundance	150 mM
6	Arginine	natural abundance	50 mM
7	Glutamic Acid	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 16 models in PDB: 2KJH, Strand ID: A Detail

Release date

2009-10-13

Citation 1

The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site
Serniwka, S.A., Shaw, G.S.
Biochemistry (2009), 48, 12169-12179, PubMed 19928833 , DOI 10.1021/bi901686j , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N resonance assignments for the human E2 conjugating enzyme, UbcH7
Serniwka, S.A., Shaw, G.S.
Biomol. NMR Assign. (2008), 2, 21-23, PubMed 19636915 , DOI 10.1007/s12104-007-9074-4 , Abstract

Hide non-primary 1 citaion

Related entities 1. UbcH8, : 1 : 1 : 8 : 287 entities Detail

Interaction partners 1. UbcH8, : 30 interactors Detail

More details for 30 interactors identified by 12 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC