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Found 1 Document (0.891 seconds)

BMRB: 16335

2KJP

Solution structure of protein YlbL (BSU15050) from Bacillus subtilis, Northeast Structural Genomics Consortium target sr713a

Authors

Liu, Y., Belote, R., Ciccosanti, C., Hamilton, K., Nair, R., Rost, B., Acton, T., Xiao, R., Swapna, G., Everett, J., Montelione, G., Prestegard, J.

Assembly

BSU15050

Entity

1. BSU15050 (polymer, Thiol state: not present), 91 monomers, 10162.34 Da Detail

NGIYASSVVE NMPAKGKIEV GDKIISADGK NYQSAEKLID YISSKKAGDK VTLKIEREEK EKRVTLTLKQ FPDEPDRAGI GVSLEHHHHH H

Formula weight

10162.34 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.8 %, Completeness: 76.7 %, Completeness (bb): 82.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.7 % (812 of 1059)	76.3 % (424 of 556)	76.3 % (313 of 410)	80.6 % (75 of 93)
Backbone	82.6 % (446 of 540)	83.3 % (155 of 186)	82.3 % (219 of 266)	81.8 % (72 of 88)
Sidechain	72.3 % (436 of 603)	72.7 % (269 of 370)	71.9 % (164 of 228)	60.0 % (3 of 5)
Aromatic	41.4 % (24 of 58)	55.2 % (16 of 29)	27.6 % (8 of 29)
Methyl	81.9 % (77 of 94)	83.0 % (39 of 47)	80.9 % (38 of 47)

1. BSU15050

NGIYASSVVE NMPAKGKIEV GDKIISADGK NYQSAEKLID YISSKKAGDK VTLKIEREEK EKRVTLTLKQ FPDEPDRAGI GVSLEHHHHH H

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity	[U-99% 13C; U-99% 15N]	0.5 mM
10	MES	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	5 mM
14	sodium azide	natural abundance	3 mM
15	D2O	natural abundance	100 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	entity	[U-99% 15N]	0.5 mM
17	MES	natural abundance	20 mM
18	sodium chloride	natural abundance	200 mM
19	calcium chloride	natural abundance	5 mM
20	DTT	natural abundance	100 mM
21	sodium azide	natural abundance	3 mM
22	H2O	natural abundance	95 %
23	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.53 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 2KJP, Strand ID: A Detail

Release date

2012-08-02

Citation

Resonance assignment protein YLBL(BSU15050), Northeast Structural Genomics Consortium Target sr713a
Liu, Y., Belote, R., Ciccosanti, C., Hamilton, K., Nair, R., Rost, B., Acton, T., Xiao, R., Swapna, G., Everett, J., Montelione, G., Prestegard, J.
Not known

Related entities 1. BSU15050, : 1 : 1 : 44 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

5 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

6 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

7 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

8 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

9 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	100 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	10 %

10 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity	[U-99% 13C; U-99% 15N]	0.5 mM
10	MES	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	5 mM
14	sodium azide	natural abundance	3 mM
15	D2O	natural abundance	100 %

11 TROSY-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	entity	[U-99% 15N]	0.5 mM
17	MES	natural abundance	20 mM
18	sodium chloride	natural abundance	200 mM
19	calcium chloride	natural abundance	5 mM
20	DTT	natural abundance	100 mM
21	sodium azide	natural abundance	3 mM
22	H2O	natural abundance	95 %
23	D2O	natural abundance	10 %

12 TROSY-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	entity	[U-99% 15N]	0.5 mM
17	MES	natural abundance	20 mM
18	sodium chloride	natural abundance	200 mM
19	calcium chloride	natural abundance	5 mM
20	DTT	natural abundance	100 mM
21	sodium azide	natural abundance	3 mM
22	H2O	natural abundance	95 %
23	D2O	natural abundance	10 %

nullKeywords mixed alpha-beta protein

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Found 1 Document (0.891 seconds)

BMRB: 16335

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. BSU15050, : 1 : 1 : 44 entities Detail

Experiments performed 12 experiments Detail

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